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| 利用分子标记辅助选择聚合Pi9(t)和Xa23基因 | |
| 引用本文： | 倪大虎,易成新,杨剑波,汪秀峰,张毅,章琦,王春连,赵开军,王文相,李莉.利用分子标记辅助选择聚合Pi9(t)和Xa23基因[J].分子植物育种,2007,5(4):491-496. |
| 作者姓名： | 倪大虎 易成新 杨剑波 汪秀峰 张毅 章琦 王春连 赵开军 王文相 李莉 |
| 作者单位： | 1. 安徽省农业科学院水稻研究所,合肥,230031 2. 中国农业科学院作物科学研究所,农作物基因资源与基因改良国农重大科学工程,北京,100081 3. 安徽省农业科学院植物保护研究所,合肥,230031 |
| 基金项目： | 农业部科技成果转化项目 , 安徽省科技攻关项目 , 安徽省自然科学基金 |
| 摘 要： | 利用分子标记辅助选择将广谱高抗稻瘟病的Pi9(t)基因和全生育期高抗白叶枯病的Xa23基因聚合到同一优良株系中,获得了含双基因的优良株系L10～L13.用来自不同地区的20个稻瘟病小种和中国流行的7个白叶枯病小种及安徽白叶枯病小种对聚合株系进行接菌鉴定,结果显示:聚合Pi9(t)和Xa23基因的株系L10～L13同时抗稻瘟病和白叶枯病;与稻瘟病的供体亲本75-1-127相比,抗性水平相当,均达抗级(R)水平,且抗谱相同;与白叶枯病的供体CBB23相比,对白叶枯病的抗性时期一致,均表现为全生育期抗性,而且抗性水平和抗谱相似.通过田间农艺性状的筛选,获得的双基因聚合系具有较好的农艺性状,可直接应用于生产或作为抗性亲本. |
| 关 键 词： | 白叶枯病 稻瘟病 Pi9(t)基因 Xa23基因 分子标记辅助选择 |
Pyramiding Pi9(t) and Xa23 Genes by Molecular Marker-assisted Selection |
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| Ni Dahu,Yi Chengxin,Yang Jianbo,Wang Xiufeng,Zhang Yi,Zhang Qi,Wang Chunlian,Zhao Kaijun,Wang Wenxiang,Li li.Pyramiding Pi9(t) and Xa23 Genes by Molecular Marker-assisted Selection[J].Molecular Plant Breeding,2007,5(4):491-496. | |
| Authors: | Ni Dahu Yi Chengxin Yang Jianbo Wang Xiufeng Zhang Yi Zhang Qi Wang Chunlian Zhao Kaijun Wang Wenxiang Li li |
| Institution: | Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031; 2 Crop Institute, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Resources and Genetic Improvement, Beijing, 100081; 3 Plant Protection Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031 |
| Abstract: | The elite lines L10 ~ L13 with Pi9(t) having wide spectrum and high resistance level and Xa23 having all-stage resistance were obtained through molecular marker-assisted selection. By artificial inoculation of 20 blast isolates, 7 Chinese epidemic bacterial blight (BB) races and Anhui epidemic BB races collected from different areas or countries, the lines confer high resistance to blast and BB. L10 ~ L13 have the similar resistance level (R) and spectrum with blast donor parent 75-1-127. Comparing with BB donor parent CBB23, the lines with two elite resistance genes have same resistance period, expressing all-stage resistance. At the same time, L10~L13 express similar resistance level and spectrum. Through agronomy selection in the field, L10 ~ L13 have better agricultural characteristics than its parents and can be used to agricultural production or as resistance materials. |
| Keywords: | Bacterial blight Blast Gene Pi9(t) Gene Xa23 Molecular market-assisted selection |
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