| 抗菌肽SMAP-29基因密码子优化及其毕赤酵母表达载体构建 |
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| 引用本文： | 任耀军,薄新文,王新华.抗菌肽SMAP-29基因密码子优化及其毕赤酵母表达载体构建[J].畜牧与兽医,2008,40(6):20-22. |
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| 作者姓名： | 任耀军 薄新文 王新华 |
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| 作者单位： | 1. 石河子大学动物科技学院,新疆,石河子,832000;新疆兵团绵羊繁育生物技术重点实验室,新疆,石河子,832000
2. 新疆兵团绵羊繁育生物技术重点实验室,新疆,石河子,832000 |
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| 基金项目： | 新疆兵团科技局基础研究项目(2006JC01). |
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| 摘 要： | 以GenBank发表的绵羊骨髓抗菌肽(SMAP-29)基因(L46854)为模板,选择毕赤酵母偏好密码子,合成SMAP-29成熟肽基因。将合成的基因克隆入pPIC 3.5K载体中构建重组酵母胞内表达载体pPIC 3.5K-SMAP-29,经酶切分析、PCR和测序鉴定证明载体构建成功。表达载体的正确构建为SMAP-29的高效表达和生物学活性研究奠定基础。
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| 关 键 词： | SMAP-29 密码子优化 毕赤酵母 载体构建 |
| 文章编号： | 0529-5130(2008)06-0020-03 |
| 修稿时间： | 2007年10月11 |
Codon optimization of SMAP-29 gene and its expression vector construction in Pichia pastoris |
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| REN Yao-jun,BO Xin-wen,WANG Xin-hua.Codon optimization of SMAP-29 gene and its expression vector construction in Pichia pastoris[J].Animal Husbandry & Veterinary Medicine,2008,40(6):20-22. |
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| Authors: | REN Yao-jun BO Xin-wen WANG Xin-hua |
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| Abstract: | A gene encoding SMAP-29(sheep myeloid antibacterial peptides-29) mature peptide was synthesized according to the published sequence(L46854) in GenBank and the biased codon usage of Pichia pastoris.The synthesized gene was inserted into Pichia pastoris expression vector pPIC3.5K,thus producing an intracellular expression plasmid pPIC3.5K-SMAP-29.Then the plasmid was identified by restriction enzyme digestion,PCR amplification and sequencing.The successfully constructed plasmid is the background for the studies on high expression and bioactivities of SMAP-29. |
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| Keywords: | SMAP-29 |
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