| 烟草甲虫高毒力苏云金杆菌的分离与筛选 |
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| 引用本文： | 李红生,张靖,高家合,李梅云,杨虹琦.烟草甲虫高毒力苏云金杆菌的分离与筛选[J].现代农业科技,2012(21):139-141,148. |
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| 作者姓名： | 李红生 张靖 高家合 李梅云 杨虹琦 |
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| 作者单位： | 湖南农业大学烟草研究院;云南省玉溪市烟草公司易门分公司;云南烟草农业科学研究院 |
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| 基金项目： | 烤烟病虫测报综防(Y10001);昆虫性诱剂在烤烟生产上的研究与应用(Y10002) |
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| 摘 要： | 对云南7个优质主产烟区收集的450份烤后烟样,运用4%NaCl培养基选择分离法,分离出苏云金芽孢杆菌475株。其中对含毒蛋白基因cryⅠ及cryⅤ的18个Bt菌株用2龄烟草甲虫进行生物毒力测定,试验9 d后,9个Bt菌株生物毒力测定校正死亡率均超过80%;试验12 d后,5个Bt菌株生物毒力测定校正死亡率均超过95%。同时对分离出的Bt菌进行PCR毒素蛋白基因鉴定,含cryⅠ毒素蛋白基因的Bt菌有7株,出菌率为1.47%;含cryⅤ毒素蛋白基因的Bt菌11株,出菌率为2.32%;没有含cryⅢ毒素蛋白基因的Bt菌。因此,为进一步用苏云金芽孢杆菌防治烟叶仓贮害虫提供了科学依据。
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| 关 键 词： | 烟草甲虫 苏云金芽孢杆菌 毒素蛋白 毒力测定 仓贮害虫 |
Isolation and Selection of High Toxicity Bacillus thuringiensis to Lasioderma serricorne |
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| Authors: | LI Hong-sheng ZHANG Jing GAO Jia-he LI Mei-yun YANG Hong-qi |
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| Institution: | 1(1 Tobacco Research Institute of Hunan Agricultural University,Changsha Hunan 410128;2 Yimen Branch of Yuxi Tobacco Company in Yunnan Province;3 Yunnan Academy of Tobacco Agriculture) |
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| Abstract: | 475 Bacillus thuringiensis strains were isolated from 450 tobacco sample collected from 7 main tobacco-produced area in Yunnan by the method of 4% NaCl cutural medium and identification of them were done by means of PCR toxin gene.The results indicated that 7 Bt strains which plating efficiency was 1.47% contained cryⅠ toxin gene,11 Bt strains contained cryⅤ gene which plating efficiency was 2.32%,there was no Bt isolates containing cryⅢ gene.The bioassay toxicity of 18 Bt isolates containing cryⅠand cryⅤ gene was determinated to Lasioderma serricorne(F.)second instar,the results showed that there existed all of 9 Bt with over 80% corrected mortality 9 days after inoculation and 12 days after that 5 isolates with over 95% corrected mortality.From these the conclusion can be drawn that Bt is potential by used to control pest on stored tobacco leaf. |
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| Keywords: | Lasioderma serricorne Bacillus thuringiensis toxin bioassay toxicity pest on stored tobacco leaf |
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