| 植物HSP70反义基因工程雄性不育表达载体的构建及鉴定 |
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| 引用本文： | 刘立科,刘根齐,侯宁,刘春光,陈建南,张文会,樊颖伦,吴冰洁. 植物HSP70反义基因工程雄性不育表达载体的构建及鉴定[J]. 农业科学与技术, 2008, 9(2): 81-84 |
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| 作者姓名： | 刘立科 刘根齐 侯宁 刘春光 陈建南 张文会 樊颖伦 吴冰洁 |
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| 作者单位： | [1]中国科学院遗传发育所,北京100101 [2]聊城大学生命科学学院,山东聊城252059 |
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| 摘 要： | 构建由水稻花药特异性启动子Osg6B驱动的植物HSP70反义表达载体，为进一步通过基因工程创制植物雄性不育奠定基础。
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| 关 键 词： | 水稻 HSP70反义基因 雄性不育 表达载体 |
Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant |
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| LIU Li-ke,LIU Gen-qi,HU Ning,LIU Chun-guang,CHEN Jian-nan,ZHANG Wen-hui,FAN Ying-lun,WU Bing-jie. Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant[J]. Agricultural Science ＆ Technology, 2008, 9(2): 81-84 |
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| Authors: | LIU Li-ke LIU Gen-qi HU Ning LIU Chun-guang CHEN Jian-nan ZHANG Wen-hui FAN Ying-lun WU Bing-jie |
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| Affiliation: | LIU Li-ke,LIU Gen-qi,H0U Ning,LIU Chun-guang,CHEN Jian-nan,ZHANG Wen-hui,FAN Ying-lun,WU Bing-jie |
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| Abstract: | [Objective] In order to study the relation between the HSP70 gene and male sterility of plant further. [Methods] Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HStrlO antisense fragment to construct HSP70 antisense expression vector. The expression vector was identified by PCR experiment and enzyme digestion. [Result]The sequence of coloned Osg6B promoter had 97% homology to the published se-quence, and the cis-regulatory element in promoter area was integrated. HSIrlO antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion. [Conclusion]The construction of expressio~a vector would lay solid foundation for utilization of genetic,engineering male sterility of plant. |
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| Keywords: | HSP70 Osg6B promoter Gene engineering Male sterility |
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