| 食品中金黄色葡萄球菌的PCR检测 |
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| 引用本文： | 刘开华,王三保,邢淑婕.食品中金黄色葡萄球菌的PCR检测[J].安徽农业科学,2007,35(2):343-344. |
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| 作者姓名： | 刘开华 王三保 邢淑婕 |
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| 作者单位： | 信阳农业高等专科学校生物工程系,河南信阳,464000;河南省食品工业科学研究所,河南郑州,450000 |
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| 摘 要： | 建立一种快速聚合酶链反应(PCR)方法,用于食品中金黄色葡萄球菌的检测.针对金黄色葡萄球菌独有的SEA基因设计1对引物,在PCR体系中对相应片断进行扩增,最后通过电泳技术与阳性对照进行对比来判断阴阳性.结果表明,该方法检出率高,样品中模板DNA含量仅有0.05 pg即可检出金黄色葡萄球菌,24 h即可报告结果.因此,PCR方法是一种高效、敏感、特异性高的检测技术,可用于食品中金黄色葡萄球菌的快速检测.
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| 关 键 词： | 聚合酶链反应 金黄色葡萄球菌 SEA基因 |
| 文章编号： | 0517-6611（2007）02-00343-02 |
| 修稿时间： | 2006-10-12 |
Detection of Staphylococcus aureus in Food by PCR |
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| LIU Kai- hua et al.Detection of Staphylococcus aureus in Food by PCR[J].Journal of Anhui Agricultural Sciences,2007,35(2):343-344. |
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| Authors: | LIU Kai- hua |
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| Institution: | Dept. of Biological Engineering, Xinyang Agricultural College, Xinyang, Henan 464000 |
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| Abstract: | Establish a rapid and sensitive method for detection of Staphylococcus aureus in food.Method A couple of primers was designed to amplify the SEA gene in Staphylococcus aureus,the amplified products were compared with the positive clones to judge the result.The limit concentration of the template DNA was 0.05 pg of food samples.And the detection results could be obtained in 24 h.Conclusion PCR was a rapid,sensitive and specific method of Staphylococcus aureus detection in food. |
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| Keywords: | Polymerase chain reaction Staphylococcus aureus SEA gene |
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