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云南地区草地贪夜蛾肠道细菌的分离及鉴定
引用本文：李青晏,唐运林,蒋睿轩,张永红,朱峰,白兴荣,顾偌铖,吴燕燕,吴玉娇,陈洁,李田,李春峰,韦俊宏,潘国庆,周泽扬.云南地区草地贪夜蛾肠道细菌的分离及鉴定[J].西南农业大学学报,2020,42(1):1-8.
作者姓名：李青晏  唐运林  蒋睿轩  张永红  朱峰  白兴荣  顾偌铖  吴燕燕  吴玉娇  陈洁  李田  李春峰  韦俊宏  潘国庆  周泽扬
作者单位：1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,5. 云南省农业科学院 蚕桑蜜蜂研究所, 云南 蒙自 661101;,5. 云南省农业科学院 蚕桑蜜蜂研究所, 云南 蒙自 661101;6. 重庆师范大学 生命科学学院, 重庆 401331,5. 云南省农业科学院 蚕桑蜜蜂研究所, 云南 蒙自 661101;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;,1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;3. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;6. 重庆师范大学 生命科学学院, 重庆 401331
基金项目：中央高校基本科研业务费团队项目（XDJK2018AA001）；中央高校基本科研业务费面上项目（XDJK2019C010）；家蚕基因组生物学国家重点实验室自设课题（2019-03）.
摘    要：草地贪夜蛾(Spodoptera frugiperda)自2019年1月入侵中国以来,现已蔓延至全国25个省份.为了解草地贪夜蛾的肠道微生物的组成,近期该课题组在云南蒙自地区的玉米地采集了草地贪夜蛾幼虫及成虫,通过分离培养结合16S rDNA测序鉴定其肠道细菌分离株的种属.在幼虫肠道中分离到32个细菌分离株,通过16S rDNA序列同源聚类可归为4个属,分别为克雷伯氏菌属(Klebsiella)、肠球菌属(Enterococcus)、沙雷氏菌属(Serratia)和摩根菌属(Morganella),其中克雷伯氏菌属(Klebsiella)丰度最高,为53.1%;在成虫肠道中分离到16个细菌分离株归为5个属,分别为不动杆菌属(Acinetobacter)、克雷伯氏菌属(Klebsiella)、微球菌属(Micrococcus)、假单胞菌属(Pseudomonas)和肠杆菌属(Enterobacter),其中不动杆菌丰度最高,为56.0%.该研究首次对入侵中国的草地贪夜蛾成虫肠道细菌进行分离鉴定,为后续研究草地贪夜蛾生物学适应性等重要问题奠定了基础.
关 键 词：草地贪夜蛾  肠道细菌  成虫  云南地区  生物适应性
收稿时间：2019/12/10 0:00:00

Isolation and Identification of Gut Bacteria of Spodoptera frugiperda Feeding on Maize in Yunnan, China
LI Qing-yan,TANG Yun-lin,JIANG Rui-xuan,ZHANG Yong-hong,ZHU Feng,BAI Xing-rong,GU Ruo-cheng,WU Yan-yan,WU Yu-jiao,CHEN Jie,LI Tian,LI Chun-feng,WEI Jun-hong,PAN Guo-qing and ZHOU Ze-yang.Isolation and Identification of Gut Bacteria of Spodoptera frugiperda Feeding on Maize in Yunnan, China[J].Journal of Southwest Agricultural University,2020,42(1):1-8.
Authors:LI Qing-yan  TANG Yun-lin  JIANG Rui-xuan  ZHANG Yong-hong  ZHU Feng  BAI Xing-rong  GU Ruo-cheng  WU Yan-yan  WU Yu-jiao  CHEN Jie  LI Tian  LI Chun-feng  WEI Jun-hong  PAN Guo-qing and ZHOU Ze-yang
Institution:1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China;,5. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101, China;,5. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101, China;,5. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China;,1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;4. School of Biotechnology, Southwest University, Chongqing 400715, China; and 1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715 China;3. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing 400715, China;6. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Abstract:Spodoptera frugiperda was first found to have invaded China in January, 2019, and has since spread to 25 provinces of China. In order to fully understand the intestinal microbiota of S. frugiperda, caterpillar and moth samples of this pest were recently collected in maize fields in Mengzi of Yunnan and their gut bacteria were isolated and cultured, and their composition was identified with 16s rDNA sequencing. A total of 32 strains were isolated from the caterpillar gut, and 16S rRNA sequence homology analysis clustered them into 4 genera, i.e. Klebsiella, Enterococcus, Serratia and Morganella. Klebsiella was the dominant genus, with an abundance ratio of 53.1%. Sixteen isolates were obtained from the moth gut, and were clustered into 5 genera, i.e. Acinetobacter, Klebsiella, Micrococcus, Pseudomonas and Enterobacter. Acinetobacter had the highest abundance (56.0%). This is the first time to isolate and report the gut bacteria of S. frugiperda moth invading China. It lays a foundation for the research on biological adaptation of S. frugiperda and other essential issues.
Keywords:Spodoptera frugiperda  gut bacteria  moth  Yunnan  biological adaptation
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