| 中国柽柳组培快繁技术研究 |
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| 引用本文： | 魏海霞,王霞,李双云,周健,王莉莉,刘德玺. 中国柽柳组培快繁技术研究[J]. 中国农学通报, 2020, 36(2): 48-52. DOI: 10.11924/j.issn.1000-6850.casb18100033 |
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| 作者姓名： | 魏海霞 王霞 李双云 周健 王莉莉 刘德玺 |
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| 作者单位： | 山东省林业科学研究院,济南 250014 |
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| 基金项目： | 山东省重点研发计划“耐盐植物对滨海盐碱地改良效应评价体系研究”(2017GNC11113)；山东省重大科技创新工程“黄河三角洲盐碱地林 业生态功能提升关键技术研究”(2017CXGC0314)；山东省林业科技创新项目“抗逆抗盐观赏植物良种选育与示范”(LYCX03-2018-14)。 |
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| 摘 要： | 为更好地对中国柽柳种质进行保存,以中国柽柳茎段为外植体进行组培快繁研究。结果表明：采取75%酒精浸泡30 s、2%次氯酸钠浸泡3~5 min和升汞浸泡5 min为最适宜的灭菌方案。MS+蔗糖20 g/L+琼脂6.5 g/L为中国柽柳最适初代培养基,MS+蔗糖30 g/L+琼脂7 g/L+6-BA 0.5 mg/L+NAA 0.1 mg/L为其最适增殖培养基,1/2MS培养基为最适生根培养基。
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| 关 键 词： | 中国柽柳 快繁 增殖 生根 培养基 |
| 收稿时间： | 2018-10-11 |
| 修稿时间： | 2019-05-21 |
Tamarix chinensis: Technique of Tissue Culture and Rapid Propagation |
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| Wei Haixia,Wang Xia,Li Shuangyun,Zhou Jian,Wang Lili,Liu Dexi. Tamarix chinensis: Technique of Tissue Culture and Rapid Propagation[J]. Chinese Agricultural Science Bulletin, 2020, 36(2): 48-52. DOI: 10.11924/j.issn.1000-6850.casb18100033 |
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| Authors: | Wei Haixia Wang Xia Li Shuangyun Zhou Jian Wang Lili Liu Dexi |
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| Affiliation: | Shandong Academy of Forestry Sciences, Jinan 250014 |
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| Abstract: | The paper aims to better preserve the germplasm of Tamarix chinensis. We took the stems of Tamarix chinensis segments as explants and carried out the tissue culture and rapid propagation. The results show that the optimum sterilization scheme is: soaked in 75% ethanol for 30 s, 2% sodium hypochlorite for 3- 5 min and mercury dichloride for 5 min. MS+sucrose 20 g/L+agar 6.5 g/L is the optimum primary medium for Tamarix chinensis, and MS+sucrose 30 g/L+agar 7 g/L+6-BA 0.5 mg/L+NAA 0.1 mg/L is the optimum subculture medium for Tamarix chinensis, 1/2MS medium is the best rooting medium. |
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| Keywords: | Tamarix Chinensis rapid propagation multiplication rooting |
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