少毛钝绥螨的生物学特性及rDNA ITS序列分析

少毛钝绥螨Proprioseiopsis asetus是在国内有分布的一种捕食螨,其研究尚不深入。为明确其生物学特性,在25℃室内条件下研究了少毛钝绥螨对芦笋蓟马1龄若虫、柑橘全爪螨低龄若螨、椭圆食粉螨1龄若螨和丝瓜花粉的食性。结果表明:少毛钝绥螨可以取食以上4种食物,其中对粉螨和芦笋蓟马的捕食量较大,对柑橘全爪螨捕食量较小。少毛钝绥螨及其近缘种的rDNA ITS序列在61~85、116~159、177~198、224~254、277~317、531~549、438~445 bp和580~594 bp等ITS区段存在差异性碱基。少毛钝绥螨及其近缘种分为两个组群。Proprioseiopsis、Neoseiulus、Amblyseius聚为一个组群(GroupⅠ),而Euseius单独聚为一个组群(GroupⅡ)。少毛钝绥螨与N.womersleyi和N.agrestis亲缘关系较近。

Biological characteristics and rDNA ITS sequence analysis of Proprioseiopsis asetus (Acari: Phytoseiidae)

Proprioseiopsis asetus(Chant)(Acari:Phytoseiidae)is a widespread predatory mite in China,and it is poorly investigated.We studied the feeding habits of P.asetus reared on the first instar nymphs of Thrips tabaci Lindeman,Aleuroglyphus ovatus Troupeau,Panonychus citri McGregor and Luffa cylindrical Roem pollen.The results showed that P.asetus could feed on the above four foods;A.ovatus and T.tabaci were the most consumed preys,and P.citri was the least consumed.The molecular identification results revealed that its ITS sequence shared an identity of 100%with that of P.asetus and it was therefore identified as P.asetus.There existed differential bases at the 61-85 bp,116-159 bp,177-198 bp,224-254 bp,277-317 bp,531-549 bp,438-445 bp and 580-594 bp fragments of ITS sequences between P.asetus and its related predatory mites.P.asetus and its related predatory mites clustered as 2 groups,in which Proprioseiopsis,Neoseiulus and Amblyseius clustered as a group(GroupⅠ),while Euseius clustered as another group(GroupⅡ).P.asetus was phylogenetically closer to N.womersleyi and N.agrestis.