华北大黑鳃金龟植物源引诱剂配方筛选及引诱效果

为研制华北大黑鳃金龟Holotrichia oblita的植物源引诱剂,选取7种植物挥发物单体,筛选各单体最佳引诱浓度并据此设计35个三元配方,通过华北大黑鳃金龟成虫触角电位反应试验及进一步的趋势行为反应试验筛选出对华北大黑鳃金龟雌、雄成虫引诱作用较强的配方,并通过笼罩引诱试验测试所筛选配方对成虫的引诱效果。结果表明,趋势行为反应试验中配方5(邻苯二甲酸二丁酯+芳樟醇+苯甲醇)、配方14(肉桂醛+苯甲醇+顺-3-己烯基乙酸酯)、配方27(1-己醇+肉桂醛+苯甲醇)和配方35(1-己醇+顺-3-己烯基乙酸酯+顺-3-己烯基异丁酸酯)对雌成虫引诱效果较好,选择反应率分别为62.09%、62.79%、60.22%和85.13%;配方3(邻苯二甲酸二丁酯+肉桂醛+顺-3-己烯基乙酸酯)、配方6(邻苯二甲酸二丁酯+芳樟醇+顺-3-己烯基乙酸酯)和配方35对雄成虫引诱效果较好,选择反应率分别为69.12%、64.22%和89.05%。笼罩引诱试验结果表明,成虫对配方3、配方6和配方35的选择反应率分别为68.16%、60.24%和70.59%,均高于其它配方;配方3、配方6和配方35引诱的成虫数量分别为61、61和72头,均显著高于其对照引诱的成虫数量(29、40和30头)。表明配方3、配方6和配方35对华北大黑鳃金龟成虫具有显著引诱效果,可作为其引诱剂备选配方应用于生态防控。

Prescription screening and trapping effect of plant volatile attractants to northern China scarab beetle Holotrichia oblita

To select the attractants of plant origin for the ecological management of northern China scarab beetle Holotrichia oblita, seven of the volatile components were selected and optimal attractant concentration was screened. Accordingly, 35 formulas consisting of three ingredients were designed, and each formula with strong attraction to female and male H. oblita adults was screened out through the measurement of antennal potential response of H. oblita adults and further behavioral trend test. The trapping effects of the selected formulas on H. oblita adults were tested by using enveloping and trapping tests. The result showed that, in the behavioral trend test, female adults were better seduced by formula 5 (dibutyl phthalate+linalool+benzyl alcohol), formula 14 (cinnamaldehyde+benzyl alcohol+(Z)-3-hexenyl acetate), formula 27 (1-hexanol+cinnamaldehyde+benzyl alcohol) and formula 35 (1-hexanol+ (Z)-3-hexenyl acetate+(Z)-3-hexenylisobutyrate), with a selection response rate of 62.09%, 62.79%, 60.22% and 85.13%, respectively; male adults were better seduced by formula 3 (dibutyl phthalate+cinnamaldehyde+(Z)-3-hexenyl acetate), formula 6 (dibutyl phthalate+linalool+(Z)-3-hexenyl acetate), and formula 35, with a selection response rate of 69.12%, 64.22% and 89.05%, respectively. The results of enveloping and trapping tests showed that the response rates of adults to formula 3, formula 6 and formula 35 were 68.16%, 60.24% and 70.59%, respectively, all greater than to other formulas. The number of trapped adults by formula 3, formula 6 and formula 35 were 61, 61 and 72 heads, respectively, significantly higher than the controls (29, 40 and 30 heads, respectively). It is concluded that formula 3, formula 6 and formula 35 had a significant trapping effect on the adult of H. oblita, and they can be used as an alternative attractant for ecological prevention and control of this pest insect.