| 橡胶树魏克汉种质群体结构分析 |
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| 引用本文： | 方家林,龙青姨,华玉伟,黄华孙. 橡胶树魏克汉种质群体结构分析[J]. 广西农业生物科学, 2013, 0(5): 627-632 |
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| 作者姓名： | 方家林 龙青姨 华玉伟 黄华孙 |
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| 作者单位： | [1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,儋州571737 [2]云南省热带作物科学研究所,景洪666100 |
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| 基金项目： | 基金项目：本研究由国家天然橡胶产业技术体系（nycytx-34）和农业部物种保护项目（NB2013-2130135和13RZZY-1）共同资助 |
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| 摘 要： | 群体结构是引起关联分析假阳性的最主要因素。本研究基于25个EST．SSRs位点，分析了283份橡胶树魏克汉种质群体的哈迪-温伯格平衡和群体分层情况。结果表明，在被检查的25个位点中13个位点偏离哈迪一温伯格平衡；在共祖系数为50％、600／0、700／0~11800／o时，283份种质中具有明确亚群归属的分别只有155份、110份、61份和22份，分别占总种质的54．78％、38．87％、21．55％和7．78％。同时，25个多态位点共形成了300个位点组合，20个位点组合（6．670／01呈显著的连锁不平衡状态，其中5个位点组合（1．67％）达到极显著连锁不平衡。由上可知283份橡胶树魏克汉种质所构成的群体具有一定的群体分层，但分化程度较小，可用于关联分析。
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| 关 键 词： | 橡胶树 魏克汉种质 EST-SSRs 群体结构 关联分析 |
Population Structure Analysis ofWickham Germplasm in Hevea brasiliensis |
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| Fang Jialin,Long Qingyi,Hua Yuwei t Huang Huasun. Population Structure Analysis ofWickham Germplasm in Hevea brasiliensis[J]. Journal of Guangxi Agricultural and Biological Science, 2013, 0(5): 627-632 |
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| Authors: | Fang Jialin Long Qingyi Hua Yuwei t Huang Huasun |
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| Affiliation: | 1.1 Key Laboratory of Biology and Genetic Resources of Rubber Tree of Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737; 2 Yunnan Institute of Tropical Crops, Jinghong, 66610 |
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| Abstract: | Population stratification is key reason resulting in false association in association analysis. Analysis of Hardy-Weinberg equilibrium and population structure on 283 Wickham germplasm was carried out by 25 EST-SSRs. The result showed that 13 of 25 EST-SSRs showed that there were Hardy-Weinberg equilibrium, and 54.78%, 38.87%, 21.55% and 7.78% of entire Wickham germplasm could be of population stratification, additionally, 20 locus combinations （6.67%） of 300 EST-SSRs combinations-derived 25 EST-SSRs were significantly linkage dis- equilibrium. So there was genetic diversity of 283 Wickham germplasm but the level of genetic diversity was lower, the population could be used for association study. |
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| Keywords: | Hevea brasiliensis Wickham germplasm EST-SSRs Population structure Association analysis |
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