| 感染水稻黑条矮缩病毒的玉米组织细胞的变化 |
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| 引用本文： | 任萍,陈丹,曹克强,路银贵,苗洪芹. 感染水稻黑条矮缩病毒的玉米组织细胞的变化[J]. 河北农业大学学报, 2009, 32(6) |
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| 作者姓名： | 任萍 陈丹 曹克强 路银贵 苗洪芹 |
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| 作者单位： | 河北农业大学,植物保护学院,河北,保定,071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北,保定,071000 |
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| 基金项目： | 河北省自然科学基金,成果转化项目 |
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| 摘 要： | 在防虫网室内,人工接种水稻黑条矮缩病毒(Rice black-streaked dwarffijivirus,RBSDV)于3叶1心期至5叶1心郑单958玉米苗上。接种10 d后,每天调查症状,抽雄期测量植株上部幼嫩茎节长度;对接种病株和未接种健株的叶片进行横切;对茎节组织进行横切和纵切,荧光染色后,显微镜下观察横切片的细胞数量、形态变化,测量茎节纵切片的细胞长度。结果显示:与未接种的健康对照相比,受侵染的玉米叶片和茎节组织的韧皮部细胞畸形、数量剧增,且排列紊乱。感病植株的节间长度缩短,细胞伸长受阻。本研究通过组织细胞形态观察,明确了RBSDV侵染玉米后,所致内部组织细胞形态的变化和外部植株矮化、叶背蜡泪状突起等典型症状的相关性。
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| 关 键 词： | 玉米粗缩病 水稻黑条矮缩病毒 细胞形态 |
Histological study on maize infected with rice black-streaked dwarf fijivirus |
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| REN Ping,CHEN Dan,CAO Ke-qiang,LU Yin-gui,MIAO Hong-qin. Histological study on maize infected with rice black-streaked dwarf fijivirus[J]. Journal of Agricultural University of Hebei, 2009, 32(6) |
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| Authors: | REN Ping CHEN Dan CAO Ke-qiang LU Yin-gui MIAO Hong-qin |
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| Affiliation: | REN Ping1,CHEN Dan2,CAO Ke-qiang1,LU Yin-gui2,MIAO Hong-qin2(1.College of Plant Protection,Agricultural University of Hebei,Baoding 071000,China,2.Institute of Plant Protection,Hebei Acad.of Agri., Forest.Sci.,Hebei Engineering Research Center for Integrated Control of Agricultural Pests,China) |
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| Abstract: | Artificial inoculation of Rice black-streaked dwarf fijivirus(RBSDV) on maize through viral vector,Laodelphax striatellus Fallen,was carried out in screen house.Symptoms were investigated after inoculation through the whole growth period and the younger stem was measured in the tasseling stage.The histological observation of the cross and longitudinal sections under fluorescence microscope of leaves and stems revealed that the phloem cells from diseased leaves and stems remarkably increased and the morpholo... |
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| Keywords: | maize rough dwarf disease rice black-streaked dwarf fijivirus cell morphology |
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