银脉单药花组织培养及植株再生

以银脉单药花(Aphelandra squarrosa)当年生幼嫩茎段为外植体,根据其茎段幼嫩程度不同分为三级(未木质化、半木质化、木质化)进行进瓶诱导并建立了无菌系;在此基础之上对其进行了丛芽增殖及生根诱导研究,实验结果表明:半木质化茎段为最佳的外植体;而且不同的外植体其最佳的灭菌条件有所不同:未木质化:φ=75%酒精30 s 1 g/L升汞5 min;半木质化:φ=75%酒精30 s 1 g/L升汞7.5 min;木质化:φ=75%酒精30 s 1 g/L升汞8.5 min;较好的增殖培养基为:MS KT0.5 mg/L IBA0.1 mg/L;较好的生根诱导培养基为:MS NAA0.5 mg/L。

Studies on Tissue Culture and Plant Regeneration of Aphelandra squarrosa

The annual tender stems of Aphelandra squarrosa,which were classified into three types(i.e.unlignified,semi-lignified and lignified tender stems),were used as explants and respectively induced into the primary culture media.Moreover,Its aseptic plantlets in vitro were obtained,and its proliferation and rooting culture were carried out.The semi-lignified tender stems of Aphelandra squarrosa were the best among the three tested explants.The results indicated that the optimal sterilization method was respectively 75% ethanol 30 s 0.1%HgCl2 5 min for the unlignified tender stems of Aphelandra squarrosa,75% ethanol 30 s 0.1% HgCl2 7.5 min for its semi-lignified tender stems,75% ethanol 30 s 0.1% HgCl2 8.5 min for its lignified tender stems among all the tested ones;the best proliferation culture medium of Aphelandra squarrosa was MS KT0.5 mg/L IBA0.1 mg/L among all the tested culture media;the optimal rooting culture medium of Aphelandra squarrosa was MS NAA0.5 mg/L among all tested culture media.