辐照花粉辅助授粉对百合远缘杂交结实的影响及杂种早期分子鉴定

研究了辐照花粉辅助授粉对百合‘pollyanna’(♀)×毛百合杂交结实的影响。结果表明:采用普通柱头授粉能获得许多膨大果实和饱满种子,表明该组合为亲合性组合。用经1000和2500Gy的60Coγ射线辐照的母本花粉进行辅助授粉明显提高了果实中饱满种子的结籽率,表明2种剂量的辐照花粉辅助授粉均能有效克服该组合的杂交障碍。结籽数的提高能获得更多的后代,增加了从F1代中选育新优单株的几率。随机选取辐照花粉辅助授粉获得的杂交子代15株,采用ISSR技术对其组培苗进行亲子鉴定,6条ISSR随机引物在15个子代中共扩增出372个条带,其中具有父本特征带114条,具有母本特征带67条,具有双亲共有带65条。ISSR鉴定结果证实了杂种的真实性。

EFFECTS OF THE IRRADIATED POLLEN ASSISTING POLLINATION TECHNIQUE ON THE SEEDSET OF LILY’S DISTANT CROSS AND EARLY MOLECULAR IDENTIFICATION OF THE HYBRIDS

The effects of the irradiated pollen assisting pollination technique on seedset of the lily 'pollyanna' (♀) ×Lilium dauricum were investigated. A number of fruits and plump seeds were produced by using the normal stigma pollination , which indicated that the cross was a compatible cross. The application of 1000 and 2500Gy irradiated pollen assisting pollination increased the seedset obviously,which showed that this method could overcome the cross barriers effectively. More seedsets means that more excellent individuals might be selected from F1 hybrids. 15 random F1individuals were selected for parentage identification by ISSR-PCR technique. Total 372 bands were generated from 6primers' ISSR-PCR amplification, in which 114 bands were the male parent characteristic bands, 67 were the female parent characteristic bands, 65 were the parents common bands, and ISSR analysis showed that they were really hybrids.