| 梅岭霉素检测方法的研究 |
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| 引用本文： | 程新,魏赛金,江莉,涂国全. 梅岭霉素检测方法的研究[J]. 江西植保, 2007, 30(3): 105-108 |
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| 作者姓名： | 程新 魏赛金 江莉 涂国全 |
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| 作者单位： | 江西农业大学生物工程系,江西,南昌330045 |
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| 基金项目： | 国家十五攻关项目(2001BA708B07—0) |
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| 摘 要： | 本文通过对梅岭霉素(Meilingmycin)的高效液相色谱法(HPLC)、紫外检测法(UV)和杀蚕生物活性检测法(BA)的研究,分别建立了梅岭霉素HPLC法、UV法和BA检测方法。通过三种检测法之间的相关性回归分析,三种检测方法相互呈直线关系。12个不同发酵单位的梅岭霉素发酵样品,分别采用HPLC法、UV法和BA法进行梅岭霉素发酵单位检测,对结果的进一步分析表明:UV法与HPLC法相比测定误差6.28%;BA法分别与HPLC法和UV法检测结果相比,其误差分别为10.48%和3.95%。
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| 关 键 词： | 南昌链霉菌 梅岭霉素 检测 |
| 文章编号： | 1006-4249（2007）03-0105-04 |
| 修稿时间： | 2007-05-10 |
The Study on Detection of Meilingmycin |
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| CHENG Xin,WEI Sai-jin,JIANG Li,TU Guo-quan. The Study on Detection of Meilingmycin[J]. Jiangxi Plant Protection, 2007, 30(3): 105-108 |
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| Authors: | CHENG Xin WEI Sai-jin JIANG Li TU Guo-quan |
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| Affiliation: | College of Biological Science and Engineering ,Jiangxi Agriculture University, Nanchang 330045 |
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| Abstract: | In this paper three methods that are UV-spectrophotometry, HPLC and BA,are employed to determine the content of Meilingmycin. Twelve samples are tested respectively by each of the three methods. The results shows that the intervelation among UV, HPLC and BA is rectilineal. It is found after a further analysis of the results that the error between UV and HPLC is 6.28%, while it is 10.48% between BA and HPLC, and 3.95% between BA and UV. |
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| Keywords: | Streptomyces nanchangsis Meilingmycin determine |
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