Antigenic analysis of feline and bovine Chlamydia psittaci with monoclonal antibodies. |
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| Authors: | H Matsuno H Fukushi T Yamaguchi K Hirai |
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| Institution: | Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan. |
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| Abstract: | Monoclonal antibodies were established for antigenic analysis of feline and bovine Chlamydia psittaci. The monoclonal antibodies recognized lipopolysaccharide (LPS), 56-64, 84 or 86 kDa antigens. At least 5 antibody-binding sites were detected on LPS with the monoclonal antibodies. The 56-64 kDa antigen was suggested to have both polypeptide and carbohydrate antibody binding sites. Immunoblotting analysis of cat and cattle sera indicated that the 56-64 kDa antigen is an important antigen in host immune response. The monoclonal antibodies are extremely useful tools to analyse the structure and function of chlamydial antigens. |
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