紫外线诱变对小麦条锈菌致病性突变的影响

为深入了解紫外线对小麦条锈菌致病性突变的影响，用不同时间的紫外线处理小麦条锈菌条中23-2，当夏孢子致死率达90%左右时确定为最适诱变剂量，为5 min。紫外线处理条中23-25min得到2个突变菌株：在尤皮Ⅱ号上表现3型的菌株和在水源11上表现2型的菌株，分别命名为尤Ⅱ-23菌株和水源11菌株，其中尤Ⅱ-23菌株经4代转接仍保持毒性，水源11菌株经两代转接后发生回复突变。尤Ⅱ-23菌株在鉴别寄主上的反应型与野生菌系相比发生了较大变化，在测试品种上的毒性范围仅次于条中32号。对野生菌系和突变菌株进行RAPD分析发现，两者间的DNA多态性存在明显差异，多态率为10.73%。为进一步研究小麦条锈病的流行规律和致病性变异机制奠定了基础。

Effects of Ultraviolet Radiation on Pathogenicty Mutation of Puccinia striiformis f. sp. Tritic

Urediospores of CY 23-2 were treated by ultraviolet with different radiation time.Five minutes were determined the proper radiation time,because the relative lethality rate of urediospores reached to 90% under this radiation dosage.Two mutant strains were obtained from urediospores of CY 23-2 which was treated 5min,named Jubilejina 2-23 isolate and Shuiyuan 11 isolate respectively.Jubilejina 2-23 isolate still kept its virulence after stabilization for 4 generations, however Shuiyuan 11 isolate occurred rev...