| HPLC测定狼牙刺不同组织器官中氧化槐果碱含量 |
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| 引用本文： | 李羽翡,吴三桥,王艳龙,刘荣堂,李新生. HPLC测定狼牙刺不同组织器官中氧化槐果碱含量[J]. 草业科学, 2009, 26(5): 62-65 |
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| 作者姓名： | 李羽翡 吴三桥 王艳龙 刘荣堂 李新生 |
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| 作者单位： | 甘肃农业大学生命科学学院,甘肃,兰州,730070;陕西省资源生物重点实验室,陕西,汉中,723000;陕西省资源生物重点实验室,陕西,汉中,723000;甘肃农业大学生命科学学院,甘肃,兰州,730070 |
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| 基金项目： | 陕西省教育厅重点实验室计划项目，陕西理工学院资助项目 |
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| 摘 要： | 建立了采用高效液相色谱法测定狼牙刺Sophra viciifolia中氧化槐果碱含量的方法：色谱柱为ZORBAX C18柱（4.6 mm×150 mm，5 μm）；流动相0.04 mol/L磷酸溶液（pH值1.8）-甲醇（体积比90∶10）；流速1.0 mL/min；检测波长220 nm；柱温20 ℃。测定了狼牙刺不同组织器官中氧化槐果碱含量，结果表明：狼牙刺植物花蕾中氧化槐果碱含量最高，达2.114%，其次是幼果、种子、花、叶、当年生幼茎，氧化槐果碱含量分别为1.735%、1.651%、1.615%、0.540%和0.244%，多年生茎含量最低，只有0.045%。该结果对狼牙刺资源的开发利用及生态保护具有积极意义。
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| 关 键 词： | HPLC 狼牙刺 氧化槐果碱 |
Determination of Oxysophocarpine in different structures of Sophora viciifolia by HPLC |
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| LI Yu-fei,WU San-qiao,WANG Yan-long,LIU Rong-tang,LI Xin-sheng. Determination of Oxysophocarpine in different structures of Sophora viciifolia by HPLC[J]. Pratacultural Science, 2009, 26(5): 62-65 |
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| Authors: | LI Yu-fei WU San-qiao WANG Yan-long LIU Rong-tang LI Xin-sheng |
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| Affiliation: | 1.College of Life Science;Gansu Agriculture University;Lanzhou 730070;China;2.Shanxi Provincial Key Laboratory of Biological Resources;Hanzhong 723000;China |
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| Abstract: | This paper established an analytical method to determine the content of oxysophocarpine in Sophora viciifolia by HPLC. ZORBAX C18（150 mm×4.6 mm,5 μm）Column and 0.04 mol/L H3PO4(pH=1.8)－CH3OH(90∶10) mobile phase were used. The identification wave length was 220 nm and column temperature was 20 ℃.The result showed that the buds were the main accumulation organs of oysophocarpine (2.114%).The contents of oxysophocarpine in other organs such as young fruit, seeds, flowers, leaves, one year growing stem and perennial stem was 1.735%、1.651%、1.615%、0.540%、0.244% and 0.045%, respectively. This result is helpful for S. viciifolia resources development and ecology protection. |
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| Keywords: | HPLC |
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