无选择标记的植物转基因方法研究技术进展

植物遗传转化中,标记基因起着非常重要的作用。目前使用的抗性标记基因大多数是抗生素或除草剂抗性基因,其引发的转基因植物安全性问题越来越受重视。笔者综述了近年来发展的各种无选择标记转基因植物方法及其在转基因植物中的应用,主要有共转化法、位点特异性重组系统、转座子系统、同源重组法和多元自主转化载体系统。通过评述各种方法的优缺点,以期推动安全型转基因植物培育和转基因植物产业化进程。

Research progress of marker-free transgenic plants

Selectable marker genes have been extraordinarily useful in genetic transformation of plants.Currently,the most frequently used selectable marker genes are antibiotic or herbicide resistance genes,which are being increasingly concerned on the safety issues of transgenic plants.In this paper,methods and applications for the cultivation of marker-free transgenic plants were reviewed,including co-transformation,site-specific recombination,transposable system,homologous recombination and multi-auto transformation system.The "pros" and "cons" of these approaches were also discussed with a view to promote the industrialization process of transgenic plants.