Structure analysis of water-soluble polysaccharide CPPS3 isolated from Codonopsis pilosula |
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| Authors: | Ya-jun Zhang Li-xia Zhang Jing-feng Yang Zhong-yan Liang |
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| Affiliation: | 1. College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;2. Department of Life Science, Daqing Normal University, Daqing 163712, China;3. College of Bio & Food Technology, Dalian Polytechnic University, Dalian, 116034, China;4. School of Life Science, Northeast Normal University, Changchun 130024, China |
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| Abstract: | Codonopsis pilosula is a perennial species of flowering plants propagated in Northeast Asia. A water-soluble polysaccharide, CPPS3, was extracted from the root of Codonopsis pilosula by boiled water extraction and ethanol precipitation. The molecular weight was estimated to be 7.4 × 104 Da determined by using Gel permeation chromatography. Monosaccharide composition and the structure of the polysaccharide were determined by gas spectroscopy, Fourier transform IR (FT-IR) spectroscopy, NMR spectroscopy and mass spectroscopy and some chemical method analysis was made. The components were galactose, arabinose and rhamnose in the molar ratio of 1.13:1.12:1. The main chain of CPPS3 is illustrated to be (1→3)-linked-β-GalpNAc, (1→3)-linked-α-Rhap and (1→2,3)-β-Galp. |
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| Keywords: | Codonopsis pilosula Polysaccharide Structure analysis |
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