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A HPTLC densitometric method for the determination of aloeverose in Aloe vera gel
Authors:Richard Lobo  Kirti S Prabhu  Arun Shirwaikar  Mamatha Ballal  C Balachandran  Annie Shirwaikar
Institution:1. Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India;2. Gulf College of Pharmacy, Ajman, United Arab Emirates;3. Department of Microbiology, International Centre, Manipal, Karnataka, India;4. Department of Skin and STD, Kasturba Medical College Hospital, Manipal, Karnataka, India
Abstract:Aloe vera gel is a familiar ingredient in a range of widely available and advertisement healthcare and cosmetic products. As to date there are no documented reports on the standardization of A. vera gel and the following study is an attempt in this direction. A. vera gel was evaluated for its aloeverose content for the first time. Aloeverose, was separated on a thin-layer of silica gel by isocratic gradient elution technique was determined by using HPTLC-photodensitometry. The proposed method is simple and sensitive and can be used for the routine assay of aloeverose in phytomedicines containing A. vera gel.
Keywords:High-performance thin-layer chromatography  Densitometry  Aloeverose  Aloe vera gel  Recovery
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