Verbascoside isolated from Tectona grandis mediates gastric protection in rats via inhibiting proton pump activity |
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Authors: | Neetu Singh Nivedita Shukla Pratibha Singh Rolee Sharma SM Rajendran Rakesh Maurya Gautam Palit |
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Institution: | 1. Division of Pharmacology, Central Drug Research Institute, CSIR, Lucknow - 226001, Uttar Pradesh, India;2. Division of Medicinal and Process Chemistry, Central Drug Research Institute, CSIR, Lucknow - 226001, U.P, India;3. Division of Botany, Central Drug Research Institute, CSIR, Lucknow - 226001, U.P, India;4. Department of Biotechnology, Integral University, Lucknow - 226026, U.P, India |
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Abstract: | Evidences have suggested that Tectona grandis (TG) attenuates gastric mucosal injury; however its mechanism has not yet been established. The aim of present study was to evaluate the gastroprotective mechanism of ethanolic extract of TG (E-EtOH), butanolic fraction (Fr-Bu) and to identify its active constituents. Anti-ulcer activities were evaluated against cold restraint (CRU) and pyloric ligation (PL) induced gastric ulcer models and further confirmed through H+ K+-ATPase inhibitory activity. Cytoprotective activity was evaluated in alcohol (AL) induced gastric ulcer model and further through PGE2 level. E-EtOH and Fr-Bu attenuated ulcer formation in CRU. Moreover E-EtOH and Fr-Bu displayed potent anti-secretory activity as evident through reduced free acidity and pepsin activity in PL, confirmed further by in vitro inhibition of H+ K+-ATPase activity. In addition cytoprotective potential of E-EtOH and Fr-Bu were apparent with protection in AL model, increased PGE2 content and enhanced mucin level in PL. Phytochemical investigations of Fr-Bu yielded terpenoides and a phenolic glycoside, verbascoside. The anti-secretory mechanism of verbascoside mediated apparently through inhibition of H+ K+-ATPase with corresponding decrease in plasma gastrin level, is novel to our finding. Gastroprotection elicited by TG might be through proton pump inhibition and consequent augmentation of the defensive mechanism. |
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Keywords: | Gastric ulcer Tectona grandis H+ K+-ATPase Verbascoside Antisecretory |
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