| 我国部分地区常见农作物上黄瓜花叶病毒分离物核酸多样性分析 |
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| 引用本文： | 陈玉珍,谭新球,朱春晖,孙书娥,刘勇,张德咏. 我国部分地区常见农作物上黄瓜花叶病毒分离物核酸多样性分析[J]. 植物保护学报, 2016, 43(3): 427-433. DOI: 10.13802/j.cnki.zwbhxb.2016.03.011 |
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| 作者姓名： | 陈玉珍 谭新球 朱春晖 孙书娥 刘勇 张德咏 |
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| 作者单位： | 1. 湖南农业大学植物保护学院,长沙410128;湖南省农业科学院植物保护研究所,园艺作物害虫治理湖南省重点实验,长沙410125;2. 湖南省农业科学院植物保护研究所,园艺作物害虫治理湖南省重点实验,长沙410125 |
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| 基金项目： | 国家自然科学基金(31171816,31171815),国家公益性行业(农业)科研专项(201303028) |
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| 摘 要： | 为明确我国黄瓜花叶病毒株系分化及系统进化基本情况,从湖南、新疆、青海和海南4省区采集1 367个样品对其进行酶联免疫和RT-PCR检测,并对分离获得的15个黄瓜花叶病毒(Cucumber mosaic virus,CMV)纯化分离物CP、MP、2b核苷酸序列进行相似性和进化树分析及生物学性状比较。结果表明,辣椒、龙葵和黄瓜的CMV阳性检出率较高,分别为54.13%、29.19%和18.46%。进化树分析显示CMV-Q5与CMV亚组II的亲缘性较高;CMV-N7为新发现的重组株系,其CP、2b基因属于CMV亚组IB,MP基因却属于CMV亚组II;其余13个分离物均属于CMV亚组IB。CMV-N7和CMV-Q5在系统寄主心叶烟和枯斑寄主苋色藜上引发的症状相似,但比对照株系CMV-P3613(IB)的发病时间要晚1~2 d,系统花叶较温和,枯斑较小。表明在以上4省区常见农作物上广泛流行的CMV存在分子变异。
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| 关 键 词： | 黄瓜花叶病毒 检测 进化 核酸多样性 |
| 收稿时间： | 2015-09-05 |
Sequence diversity analysis of Cucumber mosaic virus isolates from common crops in China |
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| Chen Yuzhen,Tan Xinqiu,Zhu Chunhui,Sun Shu''e,Liu Yong and Zhang Deyong. Sequence diversity analysis of Cucumber mosaic virus isolates from common crops in China[J]. Acta Phytophylacica Sinica, 2016, 43(3): 427-433. DOI: 10.13802/j.cnki.zwbhxb.2016.03.011 |
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| Authors: | Chen Yuzhen Tan Xinqiu Zhu Chunhui Sun Shu''e Liu Yong Zhang Deyong |
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| Affiliation: | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China;Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China,Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China,Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China,Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China,College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China;Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China;Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China |
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| Abstract: | |
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| Keywords: | Cucumber mosaic virus detection evolution sequence diversity |
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