烷化剂EMS处理水稻愈伤组织诱导突变的方法初探

采用ＥＭＳ 处理水稻体细胞愈伤组织诱导变异，试验表明，采用浸渍法处理２ｈ ，ＥＭＳ 对水稻体细胞愈伤组织的半致死质量浓度为１ ～５ ｇ／Ｌ；采用滴加法处理，每管加０ ．１ ｍｌ 时，半致死浓度为５０ ｇ／Ｌ左右。根据出苗率和变异情况，用滴加法处理，每管滴加０ ．２ ｍｌ 时，处理浓度以１ ｇ／Ｌ为好。经ＥＭＳ 处理后，再生植株株系的变异频率比对照高５ ．０２ ％ 。

Study on the Method of Mutant Induced by EMS Treating Rice Somatic Callus

The method and optimum concentration that EMS was used to treat rice somatic callus for mutant induction and some mutant lines were obtained. The result showed that the concentration of 1～5m g/L 50%LD of EMS was used to soak rice somatic calli for two hours; and in dropping method, the concentration was 1～5m g/L but 0.1ml EMS solution was added on the surface of callus every tube. According to the result of mutation of generation the concentration of 1m g/L was appropriate. The mutation ratio of generation was significantly higher than check after EMS treatment.