云锦杜鹃离体再生培养基的条件优化

[目的]研究云锦杜鹃组织培养再生植株的最佳培养基配方条件,为其快速繁殖及体细胞杂交再生植株提供基础技术参考。[方法]以云锦杜鹃幼叶为材料,接种在MS、WMP、6,7-V3种基本培养基以及不同的激素组合、培养基不同磷素用量等各种处理中,重复4次进行离体培养,筛选其脱分化和再分化效果。[结果]发现WMP有利于诱导叶出愈伤,不定芽分化最佳的基本培养基为6,7-V;继代和增殖培养基为6,7-V＋0.5mg/LZT＋0.1mg/LNAA＋0.1mg/LIAA,平均增殖系数可达12.25;试管苗最适生根培养基为1/26,7-V＋0.1mg/LNAA＋0.1mg/LIAA,30d的平均生根率达到91.20%。研究还发现,在培养基中提高磷素用量有利于再生芽的伸长。[结论]6,7-V培养基在云锦杜鹃幼叶不定芽发生中起显著作用,该研究建立了最适的云锦杜鹃离体再生体系。

Research on the Medium Optimization for the Plant Regeneration in vitro of Health Family

[Objective] The basic technical reference for the rapid propagation and the plantlet regeneration of somatic hybridization of health family-Rhododendron was provided through the study on the best medium formula for its plant regeneration be means of the system of tissue culture.[Method] The Rhododendron young leaf as explants was inoculated in vitro on the 3 basic types of media: MS,WMP and 6,7-V with the different combinations of hormones,the different amounts of phosphorus and other processing and there were four repetitions in each treatment so that the de-differentiation and re-differentiation effect from these treatments were analyzed.[Results] It was found that the medium——WMP was conducive to the callus induction from leaf; the best basic medium for the differentiation of adventitious bud was 6,7-V; the medium for its subculture and multiplication was 6,7-V + ZT 0.5 mg/L + NAA 0.1 mg/L + IAA 0.1 mg/L,with the average multiplication coefficient of up to 12.25; the best rooting medium was 1/2 6,7-V + NAA 0.1mg/L + IAA 0.1mg/L,with the average rooting rate reaching to 91.20% for 30 days’ culturing.It was also found that amount-increasing of phosphorus in the medium was favor to the elongation of regeneration bud.[Conclusion] A prominent role in induction of the adventitious buds from Rhododendron young leaf culturing was played by the medium——6,7-V and the optimal regeneration system of Rhododendron culturing in vitro was established.