^60Coγ射线对侧耳属变异株的诱变效应

应用^60Coγ射线对侧耳属变异株的双核菌丝体进行辐射诱变处理，并对其再生菌落进行菌丝形态学、细胞学、生理生化鉴定。结果发现，诱变株菌落形态发生明显变化，气生菌丝减少，生长过程减慢，细胞直径变大，分枝增多，与出发菌株拮抗线明显，EST，POD同工酶显示其酶谱差异显著，结实性研究表明，诱变株较出发株子实体分化正常，产量提高，抗性强，品质良好。显示^60Coγ射线辐射诱变是改良侧耳属菌株的有效途径。

Mutation effect ofradiation of 60 Co

The dicaryotic hyphae of the variant strain of P.ostreatus was treated by radiation of 60　Co γ ray. Mycelial morpholagical,cytological and physiological-biochemical characteristics of its mutant strain were studied.The results showed that the mutant strain had less aerial mycelium.Lower colony growth rate,bigger mycelium diameter,more branch on vegetative growth phase and had more fruitbody formation,higher yield,better quality on reproductive growth phase than the initial strain.Clear ant-agnistic line and significant differences of isoenzyme pattern of EST and POD existed between the mutant and initial strains.It showed that induced mutation from dicaryotic hyphae by 60　Co γ ray was an effective means of gaining excellent rejuvenation strain of P.ostreatus.