Isolation and characterisation of a xylanase inhibitor Xip-II gene from durum wheat |
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Authors: | Giles Elliott Anne Durand Richard K Hughes Paul A Kroon Renato D'Ovidio Nathalie Juge |
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Institution: | 1. Institute of Food Research (IFR), Norwich Research Park, Colney, Norwich NR4 7UA, UK;2. Universita degli Studi della Tuscia, Dipartimento di Agrobiologia e Agrochimica, via San Camillo de Lallis, 01100 Viterbo, Italy |
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Abstract: | Cereals contain xylanase inhibitor proteins (XIPs) which inhibit microbial xylanases from glycoside hydrolase families 10 and 11. Here, we report for the first time the isolation and characterisation of a genomic clone containing a xylanase inhibitor gene. This gene, Xip-II, isolated from a durum wheat genomic library (Triticum durum Desf.) encodes a mature protein of 307 amino acid (aa) residues that shares highest aa sequence identity (64%) with the rice RIXI xylanase inhibitor. XIP-II showed inhibition against family 11 xylanases and no chitinase activity. In silico analysis of the 5′ promoter region of Xip-II revealed sequences with similarity to known cis regulatory elements upstream from the initiation codon. In particular, the identification of a number of cis-acting elements controlling the expression of defence and seed-specific genes supports the role for this class of inhibitors in plant defence against pathogens but also provides new clues on a potential role in plant development. |
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Keywords: | Xylanase inhibitor Glycoside hydrolase family 18 Durum wheat Chitinase Pathogenesis-related proteins |
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