| 苏云金杆菌新菌株WZ-7的PCR-RFLP分析及生物活性研究 |
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| 引用本文： | 王永,王勤英,檀建新,陆秀君,李国勋. 苏云金杆菌新菌株WZ-7的PCR-RFLP分析及生物活性研究[J]. 河北农业大学学报, 2003, 26(3): 82-85 |
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| 作者姓名： | 王永 王勤英 檀建新 陆秀君 李国勋 |
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| 作者单位： | 1. 河北农业大学,植物保护学院,河北,保定,071001
2. 河北农业大学,食品科技学院,河北,保定,071001 |
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| 基金项目： | 河北省自然科学基金 (30 32 1 3)资助，河北农业大学重点科研基金(981 6计划 ) |
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| 摘 要： | 利用PCR—RFLP技术分析了自河北省土壤中分离的苏云金杆菌新菌株WZ-7，结果表明该菌株含有cryl、cry2Ab、cryllal型基因，其中cryl型基因的酶切片段类型不同于已发表的基因类型，有可能含有新的基因。SDS—PAGE分析结果出现130、79、73、66、60、58kD左右的6种晶体蛋白，毒素蛋白类型属于Ⅱ型。室内生测结果显示，该菌株对重要的农业害虫棉铃虫、甜菜夜蛾、小菜蛾、菜青虫、玉米螟等均有较高的杀虫毒力。
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| 关 键 词： | 苏云金杆菌 新菌株 WZ-7 PCR—RFLP分析 生物活性 农业害虫 毒杀作用 |
| 文章编号： | 1000-1573(2003)03-0082-04 |
| 修稿时间： | 2003-02-26 |
Analysis of cry-type gene by PCR-RFLP and toxicity activity from Bacillus thuringiensis WZ-7 |
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| WANG Yong ,WANG Qin-ying ,TAN Jian-xin ,LU Xiu-jun ,LI Guo-xun. Analysis of cry-type gene by PCR-RFLP and toxicity activity from Bacillus thuringiensis WZ-7[J]. Journal of Agricultural University of Hebei, 2003, 26(3): 82-85 |
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| Authors: | WANG Yong WANG Qin-ying TAN Jian-xin LU Xiu-jun LI Guo-xun |
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| Affiliation: | WANG Yong 1,WANG Qin-ying 1,TAN Jian-xin 2,LU Xiu-jun 1,LI Guo-xun 1 |
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| Abstract: | Cry-type genes from a new Baci llus thuringiensis WZ-7 strain was isola ted from Hebei Province were identified by PCR-RFLP.PCR-RFLP was used to dete ct the cry-type gene from Bt strains The results showed it co ntain 3 types of ICPs genes:cry1-type,cry2Ab,cry1Ia1.The enzyme-cutting result of products is difference from that of published.Cry1 -type gene may be a kind of new gene.SDS-PAGE showed that the crysta l proteins were divid ed into 130,79,73,66,60,58 kD fragments.The bioassay in lab showed WZ-7 w as high toxicity against four lepidoptera pests:Helicoverpa armigera, Spodoptera exigua,Pieris rpae,Plutella xylostella,Ostrinia furnacalis. |
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| Keywords: | Bacillus thuringiensis new iso late WZ-7 cry1 cry2 PCR-RFLP |
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