Effects of in Vitro Maturation Time of Oocytes on Sheep Nuclear Transfer Efficiency |
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| Authors: | ZHANG Xiao-jian AN Zhi-xing LI Xue-bin WANG Qing-hua FAN Cai-yun YANG Li-fen ZHANG Yong Henan Institute of Science and Technology Xinxiang |
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| Institution: | ZHANG Xiao-jian1,AN Zhi-xing1,LI Xue-bin1,WANG Qing-hua1,FAN Cai-yun1,YANG Li-fen1,ZHANG Yong2 1.Henan Institute of Science and Technology,Xinxiang 453003,2.Institute of Bioengineering,Northwest A&F University,Yangling 712100 |
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| Abstract: | Objective] The study aimed to provide references for the time of oocyte maturation in vitro and enucleation in the course of sheep nuclear transfer(NT).Method] Compared the effects of different maturation time of oocytes on enucleation efficiency and reconstructed embryo development by means of blind enucleation and fluorescence microscopy.Result] Treatment of IVM(in vitro maturation)19-21 h was significantly higher than IVM 16-18 h treatment in oocyte maturation rate(P<0.05)and was significantly higher than IVM 22-24 h treatment in enucleation rate(P<0.05).Three treatments had no significant difference in cleavage rate and blastocyst rate(P>0.05),but IVM 19-21 h treatment was significantly higher than the other 2 treatments in average cell number of blastocysts(P<0.05).Conclusion] The appropriate in vitro maturation time of oocytes was 19-21 h for sheep nuclear transfer,which could significantly improve the quality of blastocysts according to the cell number per blastocyst(P<0.05). |
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| Keywords: | Oocyte Maturation time Sheep nuclear transfer Enucleation |
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