Potato <Emphasis Type="Italic">R1</Emphasis> resistance gene confers resistance against <Emphasis Type="Italic">Phytophthora infestans</Emphasis> in transgenic tomato plants |
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Authors: | Luigi Faino Paola Carli Antonino Testa Gennaro Cristinzio Luigi Frusciante Maria Raffaella Ercolano |
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Institution: | 1.Department of Soil, Plant, Environmental and Animal Production Sciences,Federico II University of Naples,Portici,Italy;2.Laboratory of Plant Breeding,Wageningen University,Wageningen,The Netherlands;3.Department of Arboriculture, Botany, and Plant Pathology,Federico II University of Naples,Portici,Italy |
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Abstract: | Tomato is challenged by several pathogens which cause loss of production. One such pathogen is the oomycete Phytophthora infestans which is able to attack all the aerial parts of the plant. Although a wide range of resistance sources are available, genetic
control of this disease is not yet successful. Pyramiding R-genes through genetic transformation could be a straightforward
way to produce tomato and potato lines carrying durable resistance to P. infestans. In this work the R1 potato gene was transferred into tomato lines. The tomato transgenic lines were analyzed by using q-RT-PCR
and progeny segregation to determine the gene copy number. To test the hypothesis that R1 represents a specifically regulated R-gene, transgenic tomato plants were inoculated with P. infestans isolate 88133 and IPO. All the plants containing the R1 gene were resistant to the late blight isolate IPO-0 and susceptible to isolate 88133. These results provide evidence for
specific activation of the R1 gene during pathogen challenge. Furthermore, evidence for enhancement of PR-1 gene expression during P. infestans resistance response was obtained. |
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