不同库外保护时间和冷藏时间对冷藏浸酸种孵化率的影响

【目的】进一步验证库外保护时间和冷藏时间对冷藏浸酸种孵化率的影响程度。【方法】以“两广二号”正、反交一代杂交种为研究对象，将不同库外保护时间f2～7d，25℃）和不同内库冷藏时间（30～110d，3～5℃）的蚕卵，经同一标准浸酸处理后统计其冷藏浸酸种孵化率。【结果】冷藏时间相同，库外保护时间短的冷藏浸酸种比库外保护时间长的发育快，而库外保护时间间隔越长，蚕种孵化整齐度越差。随着冷藏时间的延长，不同库外保护时间的冷藏浸酸种实用孵化率提高明显，冷藏时间达到70d以上，其实用孵化率全部达到生产要求（90．0％以上）；而且不同库外保护时间的冷藏浸酸种孵化整齐度也明显提高。【结论】冷藏浸酸种的卵龄差过大、内库冷藏时间不足等容易出现孵化不整齐。因此，在生产上应尽量减少冷藏浸酸种补种，防止蚕种孵化不整齐。

Effect of protection and refrigeration time alteration on hatching rate of acid-treated silkworm(Bombyx mori)eggs

[Objective]The present experiment was aimed to investigate the effect of altering protection and refrigeration times outside storeroom on hatching rate of acid-treated silkworm （Bomhyx mori） eggs. [Method]The hatching rate of acid-treated eggs （in HC1 solution,48℃）, obtained from the cross and reciprocal cross species of silkworm variety Liangguang 2, was investigated after protecting them outside storeroom at 25℃ for 2-7 days and refrigerating at 3-5℃ for 30-110 days. [Resuh]The growth in the eggs kept for same refrigeration time was found faster in shorter protection time treated eggs compared to those kept for longer protection time. Further, the uniformity of hatching was lesser in those eggs which have been kept for longer protection time. While the practical hatching rate increased significantly in silkworm eggs kept for different protection time and the longer refrigeration duration. The hatching rate increased to more than 90% after refrigerating the eggs for 70 days, which was equal to the production demand, also the hatching uniformity of eggs was improved. [Conclusion]The age gap in silkworm eggs and insufficient refrigeration times results in irregular hatching, therefore, the pre-hatching protection and refrigeration times must be manipulated as much as possible for preventing irregular hatching uniformity of silkworm eggs.