High throughput,high resolution selection of polymorphic microsatellite loci for multiplex analysis |
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Authors: | Email author" target="_blank">Nicholas?C?CryerEmail author David?R?Butler Mike?J?Wilkinson |
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Institution: | (1) School of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6AS, UK;(2) Cocoa Research Unit, The University of West Indies, St. Augustine, Trinidad and Tobago |
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Abstract: | Background Large-scale genetic profiling, mapping and genetic association studies require access to a series of well-characterised and
polymorphic microsatellite markers with distinct and broad allele ranges. Selection of complementary microsatellite markers
with non-overlapping allele ranges has historically proved to be a bottleneck in the development of multiplex microsatellite
assays. The characterisation process for each microsatellite locus can be laborious and costly given the need for numerous,
locus-specific fluorescent primers. |
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Keywords: | |
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