| 酯酶同工酶分析鉴别生产用平菇菌种真实性研究 |
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| 引用本文： | 张庆华,赵新海,钟丽娟,徐冲. 酯酶同工酶分析鉴别生产用平菇菌种真实性研究[J]. 山东农业科学, 2009, 0(6): 10-12 |
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| 作者姓名： | 张庆华 赵新海 钟丽娟 徐冲 |
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| 作者单位： | 辽宁省微生物科学研究院,辽宁,朝阳,122000 |
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| 摘 要： | 采用垂直板聚丙烯酰胺凝胶电泳对19株生产用平菇菌种的酯酶同工酶进行了研究。共检测到16条谱带、17种酶带类型。通过聚类分析，将19株菌分成六大类群，E2（0．172）带是第一类群的特征谱带、ES（0．317）和E13（0．800）带是第三类群的特征谱带。北平4和石2026、北平1和北平9存在同种异名现象。试验结果表明，平菇酯酶多样性丰富，菌株之间的差异显著，可以作为鉴定平菇菌种真实性的依据。
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| 关 键 词： | 平菇 酯酶同工酶 聚类分析 |
Verification of Authenticity for Pleurotus ostreatus Strains by Esterase Isozyme Analysis |
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| ZHANG Qing-hua,ZHAO Xin-hai,ZHONG Li-juan,XU Chong. Verification of Authenticity for Pleurotus ostreatus Strains by Esterase Isozyme Analysis[J]. Shandong Agricultural Sciences, 2009, 0(6): 10-12 |
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| Authors: | ZHANG Qing-hua ZHAO Xin-hai ZHONG Li-juan XU Chong |
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| Affiliation: | ZHANG Qing-hua,ZHAO Xin-hai,ZHONG Li-juan,XU Chong(Liaoning Academy of Microbiological Sciences,Chaoyang 122000,China) |
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| Abstract: | The multiplicity of esterase isozyme of 19 Pleurotus ostreatus strains was studied using the vertical slab gel electrophoresis of polacrylamide in this paper.16 different bands of transport ratio and 17 types of enzyme bands were determined totally.The 19 strains were clustered into 6 large groups through the cluster analysis.The E2(0.172) band was the characteristic band of the first group.The E5(0.317) and E13(0.800) bands were the characteristic bands of the third group.Beiping4 and Shi2026,Beiping1 and ... |
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| Keywords: | Pleurotus ostreatus Esterase isozyme Cluster analysis |
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