人参的组织培养及快繁体系的建立

目的]建立人参的快繁体系。方法]应用MS培养基添加激素培养法,对人参的茎尖、根尖和幼芽进行愈伤组织诱导,并进行芽分化、芽的继代增殖和生根培养。结果]结果表明,对诱导芽分化较好的培养基是BA与NAA的组合,最佳培养基激素浓度是MS+BA2.0 mg/L+NAA0.5 mg/L。愈伤组织诱导仅出现在MS+BA1.0 mg/L+2,4-D2.0 mg/L和MS+KT0.2 mg/L+2,4-D1.0 mg/L的培养基中,但这两种培养基对芽没有诱导。以MS+BA2.0 mg/L+NAA0.5 mg/L的培养基,其继代培养效果较好。结论]采用组织培养快速繁殖人参,可有效去除病毒,增加繁殖数,确保遗传性状的稳定。

Tissue Culture and Establishment of Rapid Propagation System of Ginseng

Objective]The research aimed to establish rapid propagation system of Ginseng.Method] The stem point,the root point and the bud of ginseng were carryed on callus induction,and studying the bud differentiation,bud subculture proliferation and rooting culture with the MS culture medium added hormone.Result]The results indicated that culture medium of having better inducing bud differentiation was a combination of medium BA and NAA,but the optimal concentration of culture medium was MS BA 2.0 mg/L NAA 0.5 mg/L.Callus induction only appeared in the MS BA 1.0 mg/L 2,4-D 2.0 mg/L and MS KT 0.2 mg/L 2,4-D 1.0 mg/L of the culture medium,but they had no any action to induction of bud.The effect of subculture in MS BA 2.0 mg/L NAA 0.5 mg/L of the culture medium was better.Conclusion] Using tissue culture rapid propagation of ginseng,which can effectively remove the virus,increase the number of propagation and ensure the stability of genetic traits.