| 蝴蝶兰SRAP反应体系的建立与优化 |
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| 引用本文： | 杨珺,任羽,杨光穗,尹俊梅.蝴蝶兰SRAP反应体系的建立与优化[J].热带农业科学,2010,30(3):21-24. |
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| 作者姓名： | 杨珺 任羽 杨光穗 尹俊梅 |
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| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737;海南大学农学院,海南儋州,571737
2. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737;农业部热带作物种质资源利用重点开放实验室,海南儋州,571737 |
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| 基金项目： | 科技部国家科技支撑计划项目，海南省重大科技研发专项，海南省自然科学基金 |
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| 摘 要： | 以蝴蝶兰嫩叶提取的DNA为材料,蝴蝶兰SRAP反应体系中的重要参数Mg2＋、Taq酶、模板DNA及随机引物,建立了一套适合蝴蝶兰基因扩增的SRAP反应体系：25μL的反应体系中Mg2＋浓度为2.0mmol/L,Taq酶1.0U,DNA模板40ng,上下游引物0.8mmol/L。该体系扩增条带清晰,重复性好,有望在蝴蝶兰属植物的遗传育种研究中运用。
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| 关 键 词： | 蝴蝶兰 SRAP反应体系 优化 |
Establishment and Optimization of Sequence-Related Amplified Polymorphism System for Phalaenopsis |
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| YANG Jun,REN Yu,YANG Guangsui,YIN Junmei.Establishment and Optimization of Sequence-Related Amplified Polymorphism System for Phalaenopsis[J].Chinese Journal of Tropical Agriculture,2010,30(3):21-24. |
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| Authors: | YANG Jun REN Yu YANG Guangsui YIN Junmei |
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| Institution: | 1 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737;2 College of Agronomy,Hainan University,Danzhou,Hainan 571737;3 Ministry of Agriculture Key Laboratory of Tropical Crop Germplasm Resources Utilization,Danzhou,Hainan 571737) |
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| Abstract: | The DNA from the Phalaenopsis tender leaves was used as the experimental material.In order to establish the optimized SRAP detection system,the concentrations of Mg2＋,Taq DNA polymerase,template DNA and the random primers were optimized for SRAP-PCR reaction.The optimal conditions were 2.0 mmol/L Mg2＋,1.0 U Taq DNA polymerase,40 ng template DNA,0.8 mmol/L upstream primer and downstream primer.The amplified bands were clear and had good reproducibility,thus the optimized SRAP detection system could be used for the study on molecular markers of Phalaenopsis. |
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| Keywords: | Phalaenopsis aphrodita Rchb f SRAP reaction system optimization |
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