一个水稻生物产量突变体的遗传分析(英文)

Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. Method] In the process of screening and identification of Bar-transgenic rice,a biomass mutant was found in 10 lines of T1 progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. Result] The segregation ratio is consistent with mendelian law(3∶1). The mutant assumed not only higher plant height,wider straw and earlier florescence,but also more tillers,bigger spikes and resultantly higher biomass. PCR detections indicated that no co-segregation was observed between mutant traits and target gene(Bar) in the T-DNA inserted,proving that the mutant is not caused by the insertion of T-DNA containing target gene (Bar). Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass.

Genetic Analysis of a Biomass Mutant in Oryza sativa

Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. Method] In the process of screening and identification of Bar-transgenic rice,a biomass mutant was found in 10 lines of T1 progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. Result] The segregation ratio is consistent with mendelian law(3∶1). The mutant assumed not only higher plant height,wider straw and earlier florescence,but also more tillers,bigger spikes and resultantly higher biomass. PCR detections indicated that no co-segregation was observed between mutant traits and target gene(Bar) in the T-DNA inserted,proving that the mutant is not caused by the insertion of T-DNA containing target gene (Bar). Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass.