| Effect of Phytosulfokine-a on Agrobacterium-Mediated Transformation in Rice |
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| 引用本文： | CHEN De-xi Xu Zheng-jun MA Bing-tian LI Shi-gui. Effect of Phytosulfokine-a on Agrobacterium-Mediated Transformation in Rice[J]. 水稻科学, 2005, 12(4): 255-260 |
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| 作者姓名： | CHEN De-xi Xu Zheng-jun MA Bing-tian LI Shi-gui |
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| 作者单位： | Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China |
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| 基金项目： | ACKN0WLEDGMENTS We would like to express our sincere thanks to Professor Toshihiro Kobayashi tor providing the reagent of PSK. We are also thankful to Chen Xue-wei for giving the plasmids. This work was supported by National Transgenic Plant Research and Industrialization Program （J2000-B-012）, and the Excellent Doctor Paper Foundation of Ministry of Education, China （200054）. |
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| 摘 要： | Phytosulfokine- α （PSK- α ）, a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK- α has some influence on agrobacterium-mediated transformation in rice, PSK-α at a series of concentrations was added into co-culture medium respectively. The results showed that PSK- α indeed affected the recovery of resistant calli and the transformation frequency of rice varieties Taipei 309 and Lijiangxintuanheigu, PSK- α at the concentration of 10 nmol/L could increase induction of resistant callus and efficiency of transformation, with a 11% and 4.9% top increase, respectively than the control. However, PSK- αat 200 nmol/L could inhibit the induction of the resistant calli. Further more, the effect of PSK-α on agrobacterium-mediated transformation is related with the concentration of 2, 4-D in selection medium. Higher induction rate of resistant calli was obtained from tissues treated with PSK- α plus 2 mg/L 2, 4-D.
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| 关 键 词： | 水稻 缩氨酸 激素 抗性 分子标记 |
| 收稿时间： | 2005-06-24 |
| 修稿时间： | 2005-12-22 |
Effect of Phytosulfokine-a on Agrobacterium-Mediated Transformation in Rice |
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| CHEN;De-xi;Xu;Zheng-jun;MA;Bing-tian;LI;Shi-gui. Effect of Phytosulfokine-a on Agrobacterium-Mediated Transformation in Rice[J]. Rice science, 2005, 12(4): 255-260 |
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| Authors: | CHEN De-xi Xu Zheng-jun MA Bing-tian LI Shi-gui |
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| Abstract: | |
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| Keywords: | phytosulfokine rice Agrobacterium-mediated transformation |
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