传统SMB、Varicol和Partial-discard工艺分离纯化ECG和EGCG的比较研究

使用B型三带模拟移动床技术,以C₁₈键合硅胶为固定相,乙醇与水（20︰80,V/V）为流动相,分别采取传统SMB工艺、Varicol工艺和Partial-discard工艺分离98%的茶多酚（TP98）。以得到高纯度和高回收率的ECG和EGCG为目标,分别优化柱配置、切换时间、进样流速、冲洗流速。结果表明,采用Varicol工艺能得到纯度为91.33%的ECG,回收率为91.41%;采用Partial-discard工艺能得到纯度为90.12%的EGCG,回收率为97.83%。相对于传统模拟移动床,Varicol工艺具有提高产品纯度和回收率的优点,Partial-discard工艺具有提高收集液浓度和产品纯度的优点。

Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

With C18 bonded silica as stationary phase,ethanol and water(20︰80,V/V) as mobile phase,tea polyphenols were separated by traditional Simulated Moving Bed,Varicol process and Partial-discard process.Column configuration,switching time,feed flow rate,elution flow rate were optimized respectively in order to obtain epicatechin gallate(ECG) and epigallocatechin gallate(EGCG)with high purity and high recovery.Varicol process could be employed to separate 98% green tea polyphenols(TP98) to obtain ECG with 91.33% purity and 91.41% recovery.Partial-discard process could be adopted to separate TP98 to obtain EGCG with 90.12% purity and 97.83% recovery.Compared with traditional process,Varicol process has the advantages of higher purity and recovery of target product,while Partial-discard process shows the advantages of increasing the concentration of raffinate and the purity of target product.