| 木糖对多年生黑麦草愈伤组织生长的影响 |
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| 引用本文： | 毛萍,蒋彬,马欣荣,邓霄禹,乔定君,杨宏.木糖对多年生黑麦草愈伤组织生长的影响[J].草业科学,2011,28(5):758-762. |
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| 作者姓名： | 毛萍 蒋彬 马欣荣 邓霄禹 乔定君 杨宏 |
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| 作者单位： | 中国科学院成都生物研究所乍态恢复重点实验室,四川成都610041;中国科学院研究生院,北京100049;成都大学生物产业学院,四川成都,610106;中国科学院成都生物研究所乍态恢复重点实验室,四川成都,610041 |
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| 基金项目： | 国家863计划项目(2008AA10Z409;2009AA10Z108); 成都市科技计划项目(07GGYB212NC-010) |
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| 摘 要： | 木糖被认为是生物安全的选择试剂.为探讨木糖异构酶基因能否作为筛选标记在多年生黑麦草(Lolium perenne)遗传转化中运用.以成熟种子诱导的愈伤组织为外植体.研究木糖作为碳源对愈伤组织生长的影响,5周后观察结果.结果表明,在愈伤组织继代培养基(SM)中分别添加蔗糖、木糖以及不同配比蔗糖+木糖,愈伤组织生长状况不同...
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| 关 键 词： | 多年生黑麦草 愈伤组织 木糖 蔗糖 正向选择 生物安全 |
The effect of xylose on the callus growth of perennial ryegrass |
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| MAO Ping,JIANG Bin,MA Xin-rong,DENG Xiao-yu,QIAO Ding-jun,YANG Hong.The effect of xylose on the callus growth of perennial ryegrass[J].Pratacultural Science,2011,28(5):758-762. |
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| Authors: | MAO Ping JIANG Bin MA Xin-rong DENG Xiao-yu QIAO Ding-jun YANG Hong |
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| Institution: | MAO Ping1,2,JIANG Bin3,MA Xin-rong1,DENG Xiao-yu3,QIAO Ding-jun1,YANG Hong1 (1.The Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province,Chengdu Institute of Biology,Chinese Academy of Sciences,Sichuan Chengdu 610041,China,2.Graduate School of the Chinese Academy of Sciences,Beijing 100049,3.Faculty of Biotechnology Industry,Chengdu University,Sichuan Chengdu 610106,China) |
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| Abstract: | Xylose as a selective agent is considered to be biosafe for plant genetic transformation.For investigating the possibility of xylose isomerase gene as a selective marker in genetic transformation of perennial ryegrass(Lolium perenne),the effect of xylose on the growth of calli which derived from mature embryos of perennial ryegrass was detected.Cultured on sub-cultivation medium(SM) with different proportions of sucrose,xylose and sucrose+xylose respectively,the calli were analyzed and their weight gain wer... |
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| Keywords: | perennial ryegrass callus xylose sucrose positive selection biosafety |
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