纳米氧化锌消毒小蓟外植体应用研究

为使纳米氧化锌在组织培养中达到最佳消毒效果,以小蓟叶片外植体为材料,建立纳米氧化锌消毒的组织培养无菌体系。结果表明:纳米氧化锌浓度为1~7g·L~(-1)时,对小蓟外植体消毒效果逐渐提升,褐化率也逐渐升高,不同浓度纳米氧化锌对愈伤组织的诱导和生长没有明显的影响。纳米氧化锌最佳消毒处理浓度为5g·L~(-1),处理外植体20min。2g·L~(-1)纳米氧化锌+0.1%升汞消毒,外植体大约20%有污染,愈伤组织生长良好。3g·L~(-1)纳米氧化锌+0.1%升汞达到最佳的消毒效果,但愈伤组织诱导被抑制,生长缓慢。

Application of Nano-ZnO in Explant Sterilization of Cirsium setosum

In order to achieve the best disinfection effect of nanometer zinc oxide (Nano‐ZnO) in tissue culture , the explants of thistle leaves as materials ,the disinfecting system of Nano‐ZnO was established .Thistle ex‐plants disinfection effect and browning rate gradually rised ,under 1~7 g·L‐1 Nano‐ZnO .The best disinfection concentration of nano‐ZnO was 5 g·L‐1 and the best infection time was 20 min .Under 2 g·L‐1 Nano‐ZnO+0 .1% mercuric chloride condition ,thistle explants had about 20% pollution and callus grew well .3 g·L‐1 of nano zinc oxide + 0 .1% mercuric chloride condition could achieve the best disinfection ,but the callus induc‐tion was inhibited and grow slowly .