不同保存方法对秋石斛叶片抗寒生理指标的影响

为探讨样品的保存对其生理指标测定的影响,以秋石斛088叶片为材料,分别测定叶片和提取液在4、-20、-70℃条件下保存1、2、3d时的可溶性蛋白、叶绿素、MDA(丙二醛)及Fpro(游离脯氨酸)等4个生理指标的变化情况,研究不同生理指标测定适合的样品保存条件和时间。结果表明:叶片样品中可溶性蛋白含量随着保存时间的延长而逐渐下降,其中-70℃保存1d时含量与鲜叶相近;叶绿素含量则随着保存时间的延长呈现先上升后下降然后趋于稳定,各温度条件下叶片和提取液的叶绿素含量均在保存2d后与对鲜叶相近;MDA的含量在不同温度及时间保存处理间存在轻微的波动,但差异不显著;FPro的含量在不同温度下均随保存时间的延长均呈现骤然上升然后下降并趋于稳定,其中以提取液-20℃保存2d时与鲜叶最为接近。

Effects of Different Preserve Methods on Physiological Indexes in Leaves of Dend robium spp .

In order to discuss the effect of suitable condition and time of the sample preservation on different physiological indexes ,using leaves of Dendrobium spp .as experiment materials which were treated in 4 ,-20 and -70 ℃ under 1 ,2 and 3 days for leaf or extracting solution ,the contents of soluble protein ,chlorophyll , malondialdehyde and free proline were measured .The results showed that as preservation time extension ,the content of soluble protein decrease ,and the leaf saving in -70 ℃ for 1 day was most similar with control group .The contents of chlorophyll was first increased and then decreased finally stable with the prolonging of the storage time .And the content saving as leaf or extracting solution in -70 ℃ for 2 days was most similar with control group under treatment temperature .There was a slight fluctuation in the content of MDA in dif‐ferent temperature or time treatments ,but the difference was not significant .As preservation time extension , the content of free proline showed a sudden rise then decrease and finally stability at different temperatures treatment ,and the extracting solution saving in -70 ℃ for 2 days was most similar with control group .