乳铁蛋白的分离工艺研究

乳铁蛋白是哺乳动物乳中的生物活性物质,研究讨论了从牛初乳中分离乳铁蛋白的工艺。牛初乳经过离心脱脂得到脱脂乳,加入带羧甲基的弱酸性阳离子交换剂CM-SepharoseFastFlow在4℃下进行动态吸附,用去离子水将没有吸附上的杂蛋白冲洗掉,然后使用1.6%和5%的NaCl溶液进行阶跃洗脱;经过两步阶跃洗脱得到的乳铁蛋白纯度可达95%以上。

Study on Separating Process of Lactoferrin

The Lactoferrin is the living beings active material in the mammal mamma , and the original mainly talked about the technology of Lactoferrin departs from bovine colostrum: The bovine colostrum obtains the skimmed milk after being at odds with the community to degrease, and accedes to brining Qiang's methyl weak acid cation exchange dose a CM - Sepharose Fast Flow being living to carry on static state below 4℃s adsorbing; In the way of leave ion water shall not there is, and sundry albumen on the sticking rinses, afterwards employ the NaCl's liquor of 1.5% and 5% to carry on the stairs and jumps to wash comeing off ; After two jumps wash to come off over the Lactoferrin pureness attainability 95% of mamma that obtains.