基于无毒基因序列的稻瘟病菌指纹类型与致病型的关系初探

基于已克隆的稻瘟病菌无毒基因序列和基因组序列，设计了4对特异性引物，对不同来源的31个菌株进行PCR扩增，得到13个指纹类型。同时将31个菌株在7个中国鉴别品种、7个以丽江新团黑谷为背景的近等基因系鉴别品种和12个日本单基因鉴别品种上接种鉴定其致病型，分析菌株指纹类型与致病型之间的关系。结果表明，病菌的指纹类型与基于日本单基因鉴别品种划分的致病型间存在一定程度的对应关系。

Primary Study on Correlation Between Pathotypes and DNA Fingerprintings Based on Avirulence Gene Sequences of Rice Blast Fungus Magnaporthe grisea

According to the published sequences of avirulence genes of Magnaporthe grisea, four pairs of special primers were designed and used to amplify the types of DNA fingerprinting of 31 pathogenic isolates from various regions by PCR technique. Thirteen types of fingerprinting were obtained by assortment of the electrophoresis bands of PCR products, which showed no geographical properties except the strains from Guangdong, China. Meanwhile, the pathotypes of all isolates were detected through twice inoculation in seven Chinese differentials, seven near-isogenic lines with the genetic background of Lijiangxintuanheigu and twelve Japanese differential cultivars with a single R gene, respectively. The results showed that there were to some extent correlations between the DNA fingerprinting types of pathogen and their pathotypes based on Japanese differential cultivars.