| 利用H9亚型的HA1蛋白建立ELISA抗体检测方法 |
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| 引用本文： | 王华.利用H9亚型的HA1蛋白建立ELISA抗体检测方法[J].中国动物保健,2013(8):26-29. |
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| 作者姓名： | 王华 |
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| 作者单位： | 上海交通大学农业与生物学院上海200240 |
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| 摘 要： | 该试验对ELISA反应条件进行摸索,并确定了最适工作条件。结果表明,重组蛋白最适包被浓度为200倍稀释,最适包被条件为4℃过夜,血清稀释度为1∶50,HRP-兔抗鸡IgG的最适工作浓度为1：1000,待检血清和酶标二抗的反应时间均37℃30min,底物在室温显色10min。根据已经建立的ELISA方法及反应条件,确定阴阳性临界值为0.109。
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| 关 键 词： | H9禽流感病毒 HA1蛋白 ELISA |
Development of ELISA Antibody Test Using HA1 Protein of H9 |
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| Wang Hua.Development of ELISA Antibody Test Using HA1 Protein of H9[J].China Animal Health,2013(8):26-29. |
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| Authors: | Wang Hua |
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| Institution: | Wang Hua (Shanghai Jiao Tong University School of Agriculture And Biology,Shanghai, 200240) |
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| Abstract: | The optimal working conditions of this ELISA method was investigated and determined ： the recombinant protein diluted by 1：200, coating in the condition of 4℃ overnight; the serum dilution was 1：50 and working concentration of HRP-labeled rabbit anti-protein IgG was 1：1000, both the incubation steps above were done at 37℃ for 30min. The samples with optical dense（OD） greater than 0.109 were judged as cut off. |
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| Keywords: | H9 subtype AIV HA1 protein ELISA |
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