一种优化鸡胚成纤维细胞制备方法及生长曲线测定

鸡胚成纤维细胞（CEF）的来源方便，制备简单，耐受性好，且适合于许多病毒的生长繁殖，因此被广泛用于疫苗的生产、病毒的培养以及一些细胞和分子生物学的研究。然而传统的鸡胚成纤维细胞制备方法存在制备时间长、死细胞较多和细胞贴壁慢的问题。笔者根据经验摸索出一种实验室快速制备CEF的简便方法，并通过台盼蓝染色用细胞计数法绘制了原代CEF及次代CEF的生长曲线。该简便方法的建立为实验室快速制备高质量高纯度的鸡胚成纤维细胞提供了方便，同时对其生长曲线的测定也为进行基因转染方面的研究积累了资料。

Optimization of Preparation Method for Chicken Embryo Fibroblastand Determination of Growth Curve

Chicken embryo fibroblasts （CEF） have characteristics such as： acquirable source, easy preparation, well tolerated and suitable for many virus growth and reproduction, making them widely used in production of vac- cine, propagation of virus, and some research of cell and molecular biology. However, traditional preparation method of CEF has some faults, such as more preparation time, more dead cells and more slower cell adhesive rate and so on. Through many experience, the writer founded out a rapid preparation method of CEF. The cell was counted and its growth curve was assessed by trypan blue rejecting dyeing. The establishment of this simple method brings convenience for the Lab to rapidly prepare high purity and good quality CEF, and the determination of the cell growth curve offers new material for the gene transfection research.