| 阿月浑子的组织培养和快速繁殖(英文) |
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| 引用本文： | 于菲,苏淑钗,沈漫,姬谦龙,冷平生. 阿月浑子的组织培养和快速繁殖(英文)[J]. 农业科学与技术, 2010, 11(1): 107-108,121 |
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| 作者姓名： | 于菲 苏淑钗 沈漫 姬谦龙 冷平生 |
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| 作者单位： | 于菲,沈漫,YU Fei,SHEN Man(北京农学院植物科学技术系,北京,102206);苏淑钗,SU Shu-chai(北京林业大学林学院,北京,100083);姬谦龙,JI Qian-long(北京农学院组培中心,北京,102206:);冷平生,LENG Ping-sheng(北京农学院园林系,北京,102206) |
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| 基金项目： | 北京市自然科学基金，北京市属市管高等学教人才强教计划资助项目 |
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| 摘 要： | 以阿月浑子良种‘科尔曼’的种子和枝条作为外植体进行组织培养。结果表明:1/2DKW和1/2DKW+6-BA3.0mg/L+NAA0.05mg/L作为种子萌发和茎段分化的培养基;1/2DKW+6-BA4.0mg/L+IBA0.05mg/L为增殖培养基,增殖系数可达3.6;1/2DKW+IBA5mg/L+NAA1mg/L为生根培养基,生根率75%以上,移栽在河砂与蛭石(3∶1)混合基质中,成活率达79%以上。
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| 关 键 词： | 阿月浑子 组织培养 快速繁殖 生根 |
Micropropagation of Pistacia vera 'Kerman' |
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| YU Fei,SU Shu-chai,SHEN Man,JI Qian-long,LENG Ping-sheng. Micropropagation of Pistacia vera 'Kerman'[J]. Agricultural Science ＆ Technology, 2010, 11(1): 107-108,121 |
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| Authors: | YU Fei SU Shu-chai SHEN Man JI Qian-long LENG Ping-sheng |
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| Affiliation: | 1. Department of Plant Science and Technology Beijing University of Agriculture,Beijng 102206; 2. Department of Landscape and Gardening,Beijing University of Agriculture,Beijing 102206; 3. Center of Tissue Culture,Beijing University of Agriculture,Beijing 102206; 4. College of Forestry,Beijing Forestry University,Beijing 100083 |
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| Abstract: | [Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established. |
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| Keywords: | Pistachio (Pistacia vera L,) Tissue culture Transplant |
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