| 低温驯化过程中大青杨叶片差异蛋白质分析 |
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| 引用本文： | 李春明,白卉,于文喜.低温驯化过程中大青杨叶片差异蛋白质分析[J].东北林业大学学报,2011(10):45-49. |
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| 作者姓名： | 李春明 白卉 于文喜 |
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| 作者单位： | 林木育种国家工程实验室(北京林业大学);黑龙江省林业科学研究所;黑龙江省林业科学院; |
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| 基金项目： | 林业公益性行业科研专项(201004060); 黑龙江省森林工业总局科技计划项目(sgzjY2010012); 黑龙江省科技计划项目(GA09B202-02) |
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| 摘 要： | 为探索大青杨秋季自然降温下的低温适应机制,分别于9月8日、9月21日、10月6日3个时期对大青杨叶片总蛋白质进行提取,经双向电泳及质谱鉴定,成功鉴定45个差异表达蛋白,其中40个蛋白表达量上调2倍以上,5个蛋白表达量下调2倍以上.功能分类表明,22个蛋白与胁迫响应相关,12个与呼吸作用相关,9个与光合作用相关,5个参与...
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| 关 键 词： | 大青杨 自然降温 低温驯化 差异蛋白 |
Differential Protein in Populus ussuriensis Leaves During Natural Low-Temperature Acclimation by Proteomic Analysis |
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| Li Chunming,Beijing ,P.R.China,Bai Hui,Yu Wenxi.Differential Protein in Populus ussuriensis Leaves During Natural Low-Temperature Acclimation by Proteomic Analysis[J].Journal of Northeast Forestry University,2011(10):45-49. |
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| Authors: | Li Chunming Beijing PRChina Bai Hui Yu Wenxi |
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| Institution: | Li Chunming(National Engineering Laboratory for Tree Breeding(Beijing Forestry University),Beijing 100083,P.R.China),Bai Hui(Forestry Research Institute of Heilongjiang Province),Yu Wenxi(Heilongjiang Academy of Forestry) |
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| Abstract: | Two-dimensional gel electrophoresis(2-DE) coupled with mass spectrometry was adopted to determine the protein expression patterns of Populus ussuriensis leaves during three growth stages in order to explore the acclimation mechanism of P.ussuriensis at natural low temperature.A total of 45 differentially expressed proteins were identified,among which 40 proteins were up-regulated expression,and 5 proteins were down-regulated.Gene ontology analysis indicates that 22 proteins related to stress response,12 to ... |
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| Keywords: | Populus ussuriensis Natural low-temperature Acclimation Differential proteins |
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