| 猪产肠毒素大肠杆菌_（987）P菌毛卵黄抗体的研究 |
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| 引用本文： | 谢志恒,赛金,张开宇,张卉,王士长.猪产肠毒素大肠杆菌_（987）P菌毛卵黄抗体的研究[J].广西农业科学,2011(10):1284-1287. |
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| 作者姓名： | 谢志恒 赛金 张开宇 张卉 王士长 |
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| 作者单位： | 广西大学动物科学技术学院;河南黄淮学院; |
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| 基金项目： | 广西大学研究生创新计划项目(2007105930905M079) |
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| 摘 要： | 【目的】探索产肠毒素大肠杆菌（ETEC）987P菌株培养的最佳条件及987P菌毛诱发蛋鸡制备卵黄抗体（IgY）的最佳免疫程序,为IgY的工业化、规模化生产提供参考。【方法】扩大培养987P菌株,抽提层析纯化987P菌毛蛋白,然后以其为抗原免疫20周龄开产海兰蛋鸡,采用间接ELISA检测987P菌毛蛋白免疫IgY效价。【结果】987P菌株的最佳培养条件为37℃下Slanctz固体培养基培养24h;以纯化987P菌毛蛋白为抗原免疫20周龄开产蛋鸡,发现987P菌毛蛋白二免、三免IgY效价总体趋势基本一致,均在第3周达到峰值并维持1周,二免抗体峰值为213,三免抗体峰值是二免峰值的2倍（214）,但第4周过后三免IgY效价仍保持在213,且持续时间长,一般维持2～3周。【结论】以纯化987P菌毛蛋白为抗原免疫蛋鸡,能获得较高效价的IgY,且适当增加免疫次数能有效提高抗体水平和延长持续时间。
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| 关 键 词： | 猪 产肠毒素大肠杆菌（ETEC） 987P菌株 菌毛蛋白 卵黄抗体（IgY） |
Researchs on yolk antibody of swine enterotoxigenic Escherichia coli _(987)P pili |
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| XIE Zhi-heng ,SAI Jin,ZHANG Kai-yu,ZHANG Hui,WANG Shi-chang.Researchs on yolk antibody of swine enterotoxigenic Escherichia coli _(987)P pili[J].Guangxi Agricultural Sciences,2011(10):1284-1287. |
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| Authors: | XIE Zhi-heng SAI Jin ZHANG Kai-yu ZHANG Hui WANG Shi-chang |
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| Institution: | XIE Zhi-heng 1,SAI Jin1,ZHANG Kai-yu1,ZHANG Hui2,WANG Shi-chang 1 (1 College of Animal Sciences and Technology,Guangxi University,Nanning 530005,China,2 Huanghuai University,Zhumadian,Henan 463000,China) |
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| Abstract: | Objective]The present experiment was aimed to develop the best culture conditions for enterotoxigenic Escherichia coli(ETEC) 987P and the best immunity procedure for preparation of yolk antibody(IgY) with layer induced by 987P pili to provide reference for industrial scale production of IgY.Method]The strain 987P was cultured and its pili protein was extracted and purified.The purified pili protein was used as antigen to immune 20-week egg-production Hailan layer,and indirect ELIZA method was used for det... |
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| Keywords: | swine ETEC 987P pili protein yolk antibody |
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