Transition state stabilization by a catalytic RNA |
| |
Authors: | Rupert Peter B Massey Archna P Sigurdsson Snorri Th Ferré-D'Amaré Adrian R |
| |
Institution: | Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA. |
| |
Abstract: | The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|