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微载体悬浮培养ST细胞增殖猪瘟兔化弱毒的工艺研究
引用本文:漆世华,秦红刚,韩兴,李晶梅,熊亮,石宝兰,谢红玲.微载体悬浮培养ST细胞增殖猪瘟兔化弱毒的工艺研究[J].中国兽药杂志,2021,55(3):64-68.
作者姓名:漆世华  秦红刚  韩兴  李晶梅  熊亮  石宝兰  谢红玲
作者单位:国药集团动物保健股份有限公司,,,,,,国药集团动物保健股份有限公司
摘    要:从细胞接种密度、病毒接毒量、培养基三个方面进行研究和优化,并进行放大培养,建立了猪瘟病毒的微载体悬浮培养工艺:细胞接种密度为每个微载体15个细胞,病毒接毒量0.05 MOI,采用DMEM/F12培养基进行培养和细胞消化瓶批式消化分散细胞,培养的细胞可以完成生物反应器10 L到50 L的放大,培养的病毒含量达到7.6 l...

关 键 词:猪瘟兔化弱毒  微载体悬浮培养  ST细胞
收稿时间:2020/9/11 0:00:00
修稿时间:2021/2/26 0:00:00

Propagation of classical swine fever virus HCLV strain by microcarrier suspension culture of ST cell
QI Shi-hua,QIN Hong-gang,HAN Xing,LI Jing-mei,XIONG Liang,SHI Bao-lan,XIE Hong-ling.Propagation of classical swine fever virus HCLV strain by microcarrier suspension culture of ST cell[J].Chinese Journal of Veterinary Drug,2021,55(3):64-68.
Authors:QI Shi-hua  QIN Hong-gang  HAN Xing  LI Jing-mei  XIONG Liang  SHI Bao-lan  XIE Hong-ling
Institution:Sinopharm Animal Health Corporation Ltd.,,,,,,
Abstract:In order to develop the technology for microcarrier suspension culture of ST cells and propagation of classical swine fever virus HCLV strain in bioreactor, the cell density,amount of virus inoculation, the culture medium and Scale-up Process were studied and optimized. Results showed that the virus titerofHCLV can reached to 7.6lgTCID50/ml and more than four million RID/ml, when using the initial seeding density of 15 cells per microcarrier under the dosage of 3g /L microcarrier,DMEM/F12 medium and infected with 0.05 multiple of infection ( MOI) of HCLV. Flask batch digestion can of scale-up the cultures from 10L to 50L. All together this suspension culture process is stable and reliable, whichlaid the foundation for large scale microcarrier suspension culture of swine fever vaccine.
Keywords:microcarrier suspension culture  ST cells  HCLV
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