| 螺旋粉虱SCAR标记的建立与应用 |
| |
| 引用本文： | 张桂芬,吴霞,郭建英,刘万学,彭正强,万方浩.螺旋粉虱SCAR标记的建立与应用[J].植物保护学报,2010,37(5):385-390. |
| |
| 作者姓名： | 张桂芬 吴霞 郭建英 刘万学 彭正强 万方浩 |
| |
| 作者单位： | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 农业部外来入侵 生物预防与控制研究中心, 北京 100081;中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193;中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 农业部外来入侵 生物预防与控制研究中心, 北京 100081;中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 农业部外来入侵 生物预防与控制研究中心, 北京 100081;中国热带农业科学院环境与植物保护研究所, 儋州 571737;中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 农业部外来入侵 生物预防与控制研究中心, 北京 100081 |
| |
| 基金项目： | 国家"973"计划(2009CB119200);国家科技支撑计划(2006BAD08A14);公益性行业(农业)科研专项(200803005);国家自 然科学基金(30571254) |
| |
| 摘 要： | 螺旋粉虱Aleurodicus dispersus Russell是一种严重威胁果树、蔬菜和园林植物生产的入侵性害虫。由于该粉虱的卵和初孵若虫体型微小,与其它粉虱类害虫形态相似,且易于携带传播,难以快速准确鉴别,本研究采用特征序列扩增区域(SCAR)标记法,通过对目的片段的克隆与测序,设计螺旋粉虱特征片段扩增引物,并对该引物的种特异性和灵敏性进行检验。结果显示,利用SCAR标记法获得了螺旋粉虱特异性片段(632bp,GenBank登录号为HM240855),根据此片段的碱基序列设计1对螺旋粉虱特异性引物,其扩增片段大小为307bp;该对引物只对螺旋粉虱具有扩增能力,对近缘种、属粉虱不具有扩增效果。该引物不仅对成虫具有良好的扩增效能,对单粒卵、1~3龄若虫和拟蛹等亦具有同样的扩增能力,其最低检出阈值为1/6400头成虫。从而表明,该SCAR标记技术完全可用于螺旋粉虱的鉴定识别及其检测和监测。
|
| 关 键 词： | 螺旋粉虱 SCAR标记 分子检测 特异性扩增 口岸检疫 |
| 收稿时间： | 2010/5/22 0:00:00 |
Establishment and application of a SCAR marker for rapid diagnosis of the spiraling whitefly, Aleurodicus dispersus Russell |
| |
| Zhang Guifen,Wu Xi,Guo Jianying,Liu Wanxue,Peng Zhengqiang and Wan Fanghao.Establishment and application of a SCAR marker for rapid diagnosis of the spiraling whitefly, Aleurodicus dispersus Russell[J].Acta Phytophylacica Sinica,2010,37(5):385-390. |
| |
| Authors: | Zhang Guifen Wu Xi Guo Jianying Liu Wanxue Peng Zhengqiang and Wan Fanghao |
| |
| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100081, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100081, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100081, China;Institute of Plant and Environment Protection, Academy of Tropical Agricultural Sciences of China, Danzhou 571737, Hainan Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100081, China |
| |
| Abstract: | The spiraling whitefly,Aleurodicus dispersus Russell,is an invasive pest,which presents tremendous risk to fruit tree,vegetable and ornamental plant products in China.The egg and young nymph of the spiraling whitefly are small in size,have high degree of morphological similarity with other whitefly species and can be carried and spread easily.It is difficult to discriminate this whitefly species from others by morphological identification.In our study,a method was described for the development of DNA marker... |
| |
| Keywords: | Aleurodicus dispersus SCAR marker molecular detection specific amplification quarantine |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
| 点击此处可从《植物保护学报》下载免费的PDF全文 |
|
